文献类型：期刊文献

英文题名：Recent Progress on Enzyme Immobilization: Materials, Strategies, and Applications

作者：Wang, Shuran[1];Li, Ruoke[1];Xie, Yixuan[1];Zhang, Hanya[1];Huang, Xiaoyu[1];Chen, Siwei[1];Xu, Weixin[2];Liu, Yueling[1]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]Shanghai Univ Med & Hlth Sci, Jiading Dist Cent Hosp, Dept Lab Med, Shanghai 201800, Peoples R China

年份：2026

外文期刊名：FOOD BIOENGINEERING

收录：EI(收录号：20261920661710);WOS:【ESCI(收录号:WOS:001757944500001)】；

基金：This study was supported by the National Natural Science Foundation of China (No. 21804043) and Open Funding Project of the State Key Laboratory of Analytical Chemistry for Life Science (SKLACLS2512). The authors acknowledge the Young Interdisciplinary Innovation Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：carrier; enzyme; immobilization; immobilization strategy; matrix

摘要：Enzyme immobilization serves as a pivotal strategy for enhancing enzymatic stability, reusability, and operational performance, demonstrating significant potential across diverse fields including biocatalysis, biosensing, food processing, environmental remediation, and biomedicine. This review systematically examines recent advances in enzyme immobilization, with a focus on advanced carrier platforms such as covalent organic frameworks (COFs), metal-organic frameworks (MOFs), natural and synthetic polymers, silica materials, magnetic materials, carbon materials, and corresponding composite materials. These materials provide robust support for constructing high-performance and multifunctional immobilized enzyme systems, as their tunable porosity, rich surface chemistry, excellent biocompatibility, and unique physicochemical properties. The review critically analyzes how various immobilization strategies, such as covalent bonding, physical adsorption, coprecipitation, and encapsulation, regulate enzymatic activity, stability, and selectivity. Moreover, the characteristics of these enzyme immobilization systems such as loading capacity, operational conditions and recyclability are summarized. It highlights the remarkable performance of these systems in applications spanning chiral synthesis, sensitive biosensing, pollutant degradation, drug delivery, food and beverage processing and continuous-flow bioprocessing. Finally, the review summarizes the current research landscape and highlights the most promising innovative prospects in this rapidly evolving field of enzyme immobilization.