文献类型：期刊文献

英文题名：Efficient Synthesis of a Chiral Precursor for Angiotensin-Converting Enzyme (ACE) Inhibitors in High Space-Time Yield by a New Reductase without External Cofactors

作者：Shen, Nai-Dong[1];Ni, Yan[1];Ma, Hong-Min[1];Wang, Li-Juan[1];Li, Chun-Xiu[1];Zheng, Gao-Wei[1];Zhang, Jie[1];Xu, Jian-He[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biocatalysis & Synthet Biotechnol, Shanghai 200237, Peoples R China

年份：2012

卷号：14

期号：8

起止页码：1982

外文期刊名：ORGANIC LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000302990100009)，CCR-EXPANDED(收录号:WOS:000302990100009)】；

基金：This work was financially supported by the National Natural Science Foundation of China (No. 20902023, 31071604), Ministry of Science and Technology, P.R. China (No. 2011CB710800, 2011AA02A210), China National Special Fund for State Key Laboratory of Bioreactor Engineering (No. 2060204), and the Innovation Program of Shanghai Municipal Education Commission (No. 11CXY24).

语种：英文

摘要：A new reductase, CgKR2, with the ability to reduce ethyl 2-oxo-4-phenylbutyrate (OPBE) to ethyl (R)-2-hydroxy-4-phenylbutyrate ((R)-HPBE), an important chiral precursor for angiotensin-converting enzyme (ACE) inhibitors, was discovered. For the first time, (R)-HPBE with >99% ee was produced via bioreduction of OPBE at 1 M without external addition of cofactors. The space-time yield (700 g.L-1.d(-1)) was 27 times higher than the highest record.