文献类型：期刊文献

英文题名：Inhibition of quorum sensing in Serratia marcescens H30 by molecular regulation

作者：Zhu, H.[1,2];Shen, Y. L.[1];Wei, D. Z.[1];Zhu, J. W.[3]

机构：[1]E China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]China Univ Petr, Ctr Bioengn & Biotechnol, Qingdao 266555, Peoples R China;[3]E China Univ Sci & Technol, Chem Engn Res Ctr, Shanghai 200237, Peoples R China

年份：2008

卷号：56

期号：6

起止页码：645

外文期刊名：CURRENT MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000255751200019)】；

语种：英文

摘要：Quorum sensing in Serratia marcescens, which uses two types of signaling molecules-N-acyl homoserine lactones and furanosyl borate diester-play important regulatory roles in the synthesis of 2,3-butanediol and prodigiosin. In the hope of understanding the effect of quorum sensing on physiologic metabolism, we established two molecular strategies, one to express acyl-homoserine lactone hydrolase to inactivate AI-1 signaling molecule using an expression vector with lactose as the inducer and the other to mutate luxS gene with a suicide plasmid pUTKm2 to inhibit the synthesis of AI-2 signaling molecule.