文献类型：期刊文献

中文题名：豆油添加对尼莫克汀发酵的影响

英文题名：Effects of Soybean Oil on Streptomyces Cyaneogriseus ssp.Noncyanogenus Fermentation

作者：薛佳韵[1];赵明霞[2];王泽建[1];孙慧婕[1];王永红[1];储炬[1];庄英萍[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237;[2]苏州大学医学部药学院,江苏苏州215123

年份：2020

卷号：46

期号：4

起止页码：533

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD_E2019_2020】；

基金：国家重点研发计划项目(2017YFF0204602)。

语种：中文

中文关键词：尼莫克汀;豆油;发酵;效价

外文关键词：nemadectin;soybean oil;fermentation;titer

摘要：考察了发酵过程中豆油对尼莫克汀合成的影响,并建立了优化的豆油补加策略。研究结果表明,在5 L反应器中初始添加1.5 g/L豆油后,尼莫克汀的生物量有明显提高,与对照组相比,干重提高了29%,尼莫克汀的效价提高了27.37%,产量达到1475μg/mL,结合发酵相关参数分析,推测初始添加豆油能够有效提高蓝灰色链霉菌的初级代谢活性,并能有效提高尼莫克汀的合成速率以及延长尼莫克汀的高速合成期,从而显著提升尼莫克汀的发酵产量。
Nemadectin produced by Streptomyces cyaneogriseus ssp.noncyanogenus(S.cyaneogriseus)is a 16-membered macrocyclic lactones antibiotics,which belongs to milbemycin subfamily.Moxidectin is chemically synthesized from nemadectin,which has broad application prospects on agricultural insecticides field because of its high efficiency,safety,environmentally friendly and so on.Therefore,many researches have paid more attention in the past few decades and most of the researches were focused on genomic,chemical modification and pharmacological aspects,such as the global genome sequence ananlysis of S.cyaneogriseus,and toxicology of moxidectin on the animal deworming.However,any researches hardly focused on the fermentation technology of process optimization and regulation of nemadectin fermentation are scarce up to now.In this study,soybean oil had a positive effect nemadetin fermentation.The biomass of S.cyaneogriseus was effectively improved by adding soybean oil at start in 5 L fermentation(for about 29%),and the titer of nemadectin rised up to 1475μg/mL,which was increased by 27.37%.Based on the analysis of the fermentation parameters,The primary metabolic activity of S.cyaneogriseus and the synthesis rate of nemadectin was greatly improved by the addition of soybean oil at the start of fermentation,resulting in a significant increase in the total yield of nemadectin.