文献类型：期刊文献

英文题名：Preparation and preliminary cytocompatibility of magnesium doped apatite cement with degradability for bone regeneration

作者：Lu, Jingxiong[1];Wei, Jie[1];Yan, Yonggang[2];Li, Hong[2];Jia, Junfeng[1];Wei, Shicheng[3];Guo, Han[4];Xiao, Tiqiao[4];Liu, Changsheng[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China;[2]Sichuan Univ, Sch Phys Sci & Technol, Chengdu 610064, Peoples R China;[3]Peking Univ, Acad Adv InterDisciplinary Studies, Ctr Biomed Mat & Tissue Engn, Beijing 100871, Peoples R China;[4]Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Shanghai 201800, Peoples R China

年份：2011

卷号：22

期号：3

起止页码：607

外文期刊名：JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE

收录：;EI(收录号：20112414048062);WOS:【SCI-EXPANDED(收录号:WOS:000289302600019)】；

基金：This study was supported by grants from the National Natural Science Foundation of China (No. 30970720), The Project-sponsored by SRF for ROCS, SEM (2008), Nano special program of Science and Technology Development of Shanghai (No. 1052nm06600), Key Medical Program of Science and Technology Development of Shanghai (No. 09411954900), and the Major Program of National Natural Science Foundation of China (No. 50732002).

语种：英文

外文关键词：Apatite - Phosphatases - Hydroxyapatite - Magnesia - Setting - Cell proliferation

摘要：In the present study, we fabricated magnesium doped apatite cement (md-AC) with rapid self-setting characteristic by adding the mixed powders of magnesium oxide and calcium dihydrogen phosphate (MO-CDP) into hydroxyapatite cement (HAC). The results revealed that the md-AC with 50 wt% MO-CDP could set within 6 min and the compression strength could reach 51 MPa after setting for 1 h, indicating that the md-AC had highly initial mechanical strength. The degradability of the md-AC in Tris-HCl solution increased with the increase of MO-CDP amount, and the weight loss ratio of md-AC with 50 wt% MO-CDP was 57.5 wt% after soaked for 12 weeks. Newly flake-like apatite could be deposited on the md-AC surfaces after soaked in simulated body fluid (SBF) for 7 days. Cell proliferation ratio of MG(63) cells on md-AC was obviously higher than that of HAC on days 4 and 7. The cells with normal phenotype spread well on the md-AC surfaces and attached intimately with the substrate, and alkaline phosphatase (ALP) activity of the cells on md-AC significantly improved compared with HAC on day 7. The results demonstrate that the md-AC has a good ability to support cell proliferation and differentiation, and indicate a good cytocompatibility.