文献类型：期刊文献

英文题名：Enzyme immobilized millimeter-sized polyHIPE beads with easy separability and recyclability

作者：Wang, Mengjie[1];Wang, Meng[1];Zhu, Yun[1];Zhang, Shengmiao[1];Chen, Jianding[1]

机构：[1]East China Univ Sci & Technol, Sch Mat Sci & Engn, Shanghai Key Lab Adv Polymer Mat, Shanghai 200237, Peoples R China

年份：2019

卷号：4

期号：6

起止页码：1136

外文期刊名：REACTION CHEMISTRY & ENGINEERING

收录：;EI(收录号：20192407032443);WOS:【SCI-EXPANDED(收录号:WOS:000469257900013)】；

基金：This work was supported by the National Natural Science Fund of China (51773059), the Natural Science Foundation of Shanghai (16ZR1407800), and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Acrylic monomers - Amino acids - Enzymes

摘要：Enzyme functionalized porous beads were fabricated through covalently immobilizing Candida antarctica lipase B (CALB) onto high internal phase emulsion (HIPE) templated poly.glycidyl methacrylate) (PGMA) beads. These millimeter-sized beads were prepared by photopolymerizing the continuous phase of HIPE drops and then removing the dispersed phase. By covalently coupling the lysine residues of CALB to the epoxy groups from GMA, a series of enzyme immobilized polyHIPE beads were produced, with an enzyme loading of up to 0.18 g g(-1) carrier. The resulting immobilized enzyme was highly stable to temperature and storage and could be recycled multiple times. The immobilized enzyme maintained 85% residual activity after 5 reaction cycles and 60% residual activity after 30 days of storage. The enzymatic esterification between hexanol and hexyl hexanoate had a conversion rate of up to 95%, showing that the enzyme immobilized beads were a good enzyme catalyst.