文献类型：期刊文献

英文题名：Purification, structure identification and immune activity of a neutral polysaccharide from Cynanchum Auriculatum

作者：Wang, Yilin[1];Han, Jin[2];Yue, Yun[1];Wu, Yuze[1];Zhang, Wenqing[1];Xia, Wei[1,3];Wu, Mengqi[1,3]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Bright Dairy & Food Co Ltd, Shanghai Engn Res Ctr Dairy Biotechnol, Dairy Res Inst, State Key Lab Dairy Biotechnol, Shanghai 200436, Peoples R China;[3]130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2023

卷号：237

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20242616330369);WOS:【SCI-EXPANDED(收录号:WOS:000966686600001)】；

基金：This work was supported by the Scientific Fund (19DZ2281400) of Shanghai Engineering Research Center of Dairy Biotechnology.

语种：英文

外文关键词：Cynanchum Auriculatum; Neutral polysaccharide; Degradation of starch; Structure identification; Immune activity

摘要：The crude polysaccharides CAPS and CAP of Cynanchum Auriculatum, which were prepared by degrading starch by single-enzymatic method (alpha-amylase) and double-enzymatic method (alpha-amylase and glucoamylase) respec-tively, were compared. CAP had good water solubility and higher non-starch polysaccharide content. A homo-geneous neutral polysaccharide CAP-W, with the degree of acetylation about 17 %, was obtained from CAP by anion exchange column chromatography. Its detailed structure was identified by various methods. CAP-W, with the weight average molecular weight of 8.4 kDa, was composed of mannose, glucose, galactose, xylose, and arabinose in a molar ratio of 1.27:1.00:0.25:0.10:1.16. The backbone included fl-1,4-Manp, fl-1,4,6-Manp, fl-1,4-Glcp and fl-1,4,6-Glcp residues, with branches at the O-6 position of fl-1,4,6-Manp and fl-1,4,6-Glcp residues, consisting of a-T-Araf, a-1,5-Araf, a-1,2,5-Araf, a-1,3,5-Araf, T-Xylp,1,4-Xylp, fl-T-Manp and fl-T-Galp residues. In vitro immunological experiments suggested that CAP-W improved the phagocytic ability of macrophages, stimulated the release of NO, TNF-alpha and IL-6 from RAW264.7 cells, promoted the expression of NF-kappa B and caused nuclear translocation of NF-kappa B p65.