文献类型：期刊文献

中文题名：核苷磷酸化酶的产酶条件优化的研究

英文题名：Study on Optimal Conditions of NPase

作者：闫蓬勃[1];邱蔚然[1];丁庆豹[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2001

卷号：8

期号：2

起止页码：83

中文期刊名：药物生物技术

外文期刊名：Pharmaceutical Biotechnology

收录：CSTPCD;;Scopus;CSCD:【CSCD_E2011_2012】；

语种：中文

中文关键词：乙酰短杆菌;核苷磷酸化酶;5-氟尿苷;产酶条件

外文关键词：Brevibacterium acetylicum, Nucleoside phosphorylase, 5-Fluorouridine

摘要：以鸟苷和 5 氟尿嘧啶为底物 ,乙酰短杆菌菌体为酶源酶法合成 5 氟尿苷。采用酵母浸膏培养基(酵母浸膏 4.0 % ,葡萄糖 0 .4% ,NaCl 0 .3 % ,pH7.0 ) ,较高的溶氧量 ,同时添加 0 .6 %的豆油 ,培养 12h ,产酶量可提高 30 %。
5-Fluorouride was enzymatically synthesized from guanosine and 5-fluorouracil by brevibacterium acetylicum The suitable culture medium contained 40g yeast extract, 4g glucose, 3g NaCl in 1 liter while pH was adjusted to 7 0 with NaOH After brevibacterium acetylicum was cultured for 12 h in the condition of the higher dissolve oxygen and 0 6% soybean oil supplied , the amount of enzym produced can be increased by 30%