文献类型：期刊文献

英文题名：Construction of an off-line two dimensional reversed-phase liquid chromatography/ultra-high performance supercritical fluid chromatography method for rapid and comprehensive analysis of Piper kadsura

作者：Xin, Huaxia[1];Fu, Qing[1];Yuan, Yun[1];Liu, Yanfang[2];Ke, Yanxiong[1];Jin, Yu[1];Liang, Xinmiao[1,2]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Nat Med, Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning, Peoples R China

年份：2017

卷号：127

起止页码：9

外文期刊名：JOURNAL OF SUPERCRITICAL FLUIDS

收录：;EI(收录号：20171403519215);WOS:【SCI-EXPANDED(收录号:WOS:000405879400002)】；

基金：This work was supported by the Project of National Natural Science Foundation of China (21305138).

语种：英文

外文关键词：Ultra-high performance supercritical fluid; chromatography; Reversed-phase liquid chromatography; Two-dimensional chromatography; Rapid analysis; Piper kadsura

摘要：In this study, ultra-high performance supercritical fluid chromatography(UHPSFC) was chosen to construct an off-line two dimensional chromatography with reversed-phase liquid chromatography (RPLC) for rapid and comprehensive analysis of Piper kadsura. The sample was firstly divided into a petroleum ether (PE) extract and an ethyl acetate (EA) extract, and then they were separated into 21 and 28 fractions in the first dimensional RPLC, respectively. In the second dimension, the effect of UHPSFC parameters on the analysis speed was investigated. Each PE fraction could be analyzed in 2.0min, and every EA fraction could be analyzed in 3.0 min. In total, at least 1033 peaks were detected by this method, including 204 peaks from the PE fractions and 829 peaks from the EA fractions. The 2D-RPLC/UHPSFC system with the characteristics of fast separation and high orthogonality has a great potential for the rapid and comprehensive analysis of the complex samples. (C) 2017 Elsevier B.V. All rights reserved.