文献类型：期刊文献

中文题名：蛋白质芯片分析在呋喃唑酮检测中的应用研究

英文题名：Research of residues of furazolidone by protein chip

作者：金娜[1];叶邦策[2]

机构：[1]温州出入境检验检疫局,浙江温州325000;[2]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2011

卷号：32

期号：9

起止页码：423

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：蛋白质芯片;AOZ;检测

外文关键词：protein chip; AOZ; detection

摘要：目的:研究了蛋白质芯片技术应用于呋喃唑酮代谢物(AOZ)检测的可行性。方法:以免疫竞争原理建立蛋白芯片体系,通过各项影响因素的优化,得出了稳定的竞争曲线。结果:该蛋白芯片能实现对AOZ高通量的快速、准确的检测,检测限达到0.1ng/mL。结论:蛋白质芯片技术应用于食品抗生素检测有独特的优势,可以在该领域获得广泛的应用。
Objective：The feasibility of the protein biochip technology for the detection of the AOZ was studied.Methods：Protein biochip by indirect competitive fluorescence immunoassay was established.Through the optimization of various factors,competition curve was obtained.Results：The protein chip can achieve high throughput and accurate detection of AOZ.The detection limit is 0.1ng/mL.Conclusion：For the simplicity and flexibility,it can be used as fast,high throughput determination method for the other contamination in foods or environment.