文献类型：期刊文献

英文题名：High-Yield Biosynthesis of 3-Hydroxypropionic Acid from Acetate by Engineering Bicarbonate Supply and Malonyl-CoA Partitioning in Escherichia coli

作者：He, Junchen[1];Luo, Yuanchan[1];Wu, Hui[1,2]

机构：[1]East China Univ Sci & Technol, China Natl Light Ind Council, Shanghai Collaborat Innovat Ctr Biomfg Technol, Key Lab Biobased Mat Engn,Sch Biotechnol, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Dalian Univ Technol, Sch Bioengn, Key Lab Biointelligent Mfg, MOE, 2 Linggong Rd, Dalian 116000, Peoples R China

年份：2026

卷号：14

期号：27

起止页码：12543

外文期刊名：ACS SUSTAINABLE CHEMISTRY & ENGINEERING

收录：;EI(收录号：20262921128639);Scopus(收录号：2-s2.0-105044961570);WOS:【SCI-EXPANDED(收录号:WOS:001810437600001)】；

基金：This study was supported by the National Key R&D Program of China (2025YFA0922200), Science and Technology Commission of Shanghai Municipality (24HC2820800), and Fundamental Research Funds for the Central Universities (DUT24RC(3)029).

语种：英文

外文关键词：3-hydroxypropionic acid; acetate; Escherichia coli; metabolic engineering; malonyl-CoA pathway; fed-batch fermentation

摘要：3-Hydroxypropionic acid (3-HP) is a versatile platform chemical with broad applications in biodegradable plastics and superabsorbent polymers. Acetate derived from waste biomass and industrial off-gases can serve as a sustainable C2 feedstock for 3-HP production. However, the efficient conversion of acetate to 3-HP remains constrained by insufficient intracellular bicarbonate supply and competition with fatty acid biosynthesis for malonyl-CoA. To address these issues, carboxylation efficiency was enhanced in E. coli by co-expressing carbonic anhydrase (CA) with sodium-dependent bicarbonate transporters (BicA) and supplementation with sodium bicarbonate. Further overexpression of fabF was used to inhibit the initiation of fatty acid biosynthesis, reducing competition for malonyl-CoA. The engineered strain achieved a 3-HP yield of 0.73 g/g in shake flask culture, approaching the theoretical maximum without chemical inhibitors. In a fed-batch bioreactor, the 3-HP titer reached 20.55 g/L with a yield of 0.56 g/g (0.37 mol/mol), corresponding to 74.67% of the theoretical maximum yield. This bioprocess enables efficient valorization of sustainable C2 feedstocks, offering a promising pathway for the industrial production of bio-based platform chemicals.