文献类型：期刊文献

中文题名：Real-time and on-line monitoring of ethanol fermentation process by viable cell sensor and electronic nose

作者：Yao Feng[1];Xiwei Tian[1];Yang Chen[1];Zeyu Wang[1];Jianye Xia[1];Jiangchao Qian[1];Yingping Zhuang[1];Ju Chu[1]

机构：[1]State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,130 Meilong Road,P.O.Box 329#,Shanghai,200237,China

年份：2021

卷号：8

期号：1

起止页码：1405

中文期刊名：Bioresources and Bioprocessing

外文期刊名：生物资源与生物加工(英文)

基金：The National Key Research and Development Program(2017YFB0309302);the Fundamental Research Funds for the Central Universities(WF1814032).

语种：英文

中文关键词：Ethanol;On-line;Viable cell sensor;Electronic nose

摘要：In this study,introduction of a viable cell sensor and electronic nose into ethanol fermentation was investigated,which could be used in real-time and on-line monitoring of the amount of living cells and product content,respectively.Compared to the conventional off-line biomass determination,the capacitance value exhibited a completely consistent trend with colony forming units,indicating that the capacitance value could reflect the living cells in the fermentation broth.On the other hand,in comparison to the results of off-line determination by high-performance liquid chromatography,the ethanol concentration measured by electronic nose presented an excellent consistency,so as to realize the on-line monitoring during the whole process.On this basis,a dynamic feeding strategy of glucose guided by the changes of living cells and ethanol content was developed.And consequently,the ethanol concentration,productivity and yield were enhanced by 15.4%,15.9% and 9.0%,respectively.The advanced sensors adopted herein to monitor the key parameters of ethanol fermentation process could be readily extended to an industrial scale and other similar fermentation processes.