文献类型：期刊文献

中文题名：北冬虫夏草深层发酵高产虫草素工艺的优化

英文题名：Optimization of submerged fermentation process of Cordyceps militaris to produce a high cordycepin content

作者：刘艳芳[1];唐庆九[1];顾俊杰[1];张劲松[1];杨焱[1];冯娜[1];周帅[1];魏东芝[2]

机构：[1]国家食用菌工程技术研究中心 农业部应用真菌资源与利用重点开放实验室；上海市农业遗传育种重点开放实验室；上海市农业科学院食用菌研究所,上海201106;[2]华东理工大学生物反应器工程国家重点实验室,鲁华生物技术研究所,上海200237

年份：2010

卷号：26

期号：3

起止页码：26

中文期刊名：上海农业学报

外文期刊名：Acta Agriculturae Shanghai

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：上海市农委重点攻关项目[沪农科攻字（2009）第2-2号）]资助

语种：中文

中文关键词：北冬虫夏草;虫草素;深层发酵;培养方式

外文关键词：Cordyceps rnili taris ; Cordycepin; Submerged fermentation Culture conditions

摘要：对23个北冬虫夏草菌株发酵液中虫草素的含量进行了测定，发现发酵液中虫草素含量最高的菌株为21号；对该菌株的发酵条件进行优化，获得最适培养基配方为：蔗糖40g／L，蛋白胨15g／L，MgSO4·7H2O0．5g／L，KH2PO40．5g／L，K2HPO4·3H2O 0．5g／L，pH自然；在此培养基中添加0．5g／L腺嘌呤进行培养，可使发酵液中虫草素含量提高约30％，同时腺嘌呤的添加可以加快虫草素的合成。培养方式的考察结果表明：纯静置培养有利于虫草素的产生和积累，静置培养28d，所得发酵液中虫草素含量达2．05g／L，比优化前提高了2倍。
The fermented broths of 23 kinds of Cordyceps militaris strains were compared in terms of cordycepin content and it was found that Strain 21＇s fermented broth was the highest in cordycepin content. Optimization of the fermentation conditions for Strain 21 showed that the optimal medium was 40 g/L sucrose, 15 g/L peptone,0.5 g/L MgSO4-7H2O,0.5 g/L KH2PO4 and 0.5 g/L K2HPO4- 3H20,and its pH value was natural. Culture with this medium to which 0.5 g/L adenine was added could make the cordycepin content of the fermented broth increase by 30%. It was proved that the added adenine could accelerate the synthesis of cordycepin. The investigation of the culture conditions showed that static culture was beneficial to the synthesis and accumulation of cordycepin, and 28 d static culture could gain 2.05 g/L cordycepin in the fermented broth which was two times higher than that before optimization.