文献类型：期刊文献

英文题名：Whole-cell catalytic synthesis of cadaverine by recombinant Corynebacterium glutamicum using corncob residue as carbohydrate feedstock

作者：Xu, Ying-Ying[1];Zhang, Bin[1];Bao, Jie[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2025

卷号：220

外文期刊名：BIOCHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20251718282542);WOS:【SCI-EXPANDED(收录号:WOS:001479982600001)】；

基金：This research was supported by the National Key R & D Program of China (2023YFA0914400) , the National Natural Science Foundation of China (32301269) , China Postdoctoral Science Foundation (2023M741175) , and the Yangfan Project of Science and Technology Committee of Shanghai Municipality (23YF1409900) .

语种：英文

外文关键词：Cadaverine; Corynebacterium glutamicum; Whole-cell catalysis; Corncob residue; Lysine

摘要：Industrial production of cadaverine primarily relies on whole-cell catalysis of lysine using engineered Escherichia coli as host cells. The growing interest in cadaverine as a monomer of biobased polyamides requires inexpensive, non-food carbohydrate feedstock for its bioproduction, rather than using food-derived sugars as feedstock. This study used corncob residue as the starting carbohydrate feedstock followed by enzymatic hydrolysis to obtain the sugars. Since E. coli was not tolerant to even minor inhibitor residues in corncob residue hydrolysate, the robust Corynebacterium glutamicum was used as the host bacterium followed by the metabolic modifications of secretory expression of lysine decarboxylase via Ncgl1289 and cgR_0949, and cadaverine degradation pathway knockout. The final whole-cell catalysis of C. glutamicum recombinant using corncob hydrolysate as carbohydrate feedstock achieved a record-high 78.19 g/L of cadaverine with a conversion yield of 91 %.