文献类型：期刊文献

英文题名：Visual diagnostic of Helicobacter pylori based on a cascade amplification of PCR and G-quadruplex DNAzyme as a color label

作者：Liu, Zhanmin[1];Yao, Chenhui[1];Wang, Yanming[1];Zheng, Wenyun[2]

机构：[1]Shanghai Univ, Sch Life Sci, Shanghai 200444, Peoples R China;[2]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China

年份：2018

卷号：146

起止页码：46

外文期刊名：JOURNAL OF MICROBIOLOGICAL METHODS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000428490300010)】；

基金：This work was supported by the National Natural Science Foundation of China (Nos. 31071545).

语种：英文

外文关键词：Visual diagnostic; Helicobacter pylori; G-quadruplex DNAzyme

摘要：Helicobacter pylori is a spiral-shaped, Gram-negative, microaerophilic and fastidious bacterium. It is the main cause of chronic gastritis as well as gastric and duodenal ulcers. The diagnosis of H. pylori infection is significant for the selection of therapy and for the follow up of eradication success. A simple and robust strategy based on the cascade of PCR and DNAzyme catalyzed reaction was utilized to detect H. pylori. The design of the primer pair would enable PCR to synthesize aptamer of DNAzyme at the 3' end of PCR products. G-quadruplex DNAzyme as a color label can exhibit peroxidase-like activity to amplify the specific signal and demonstrate a colorimetric signal to indicate the diagnostic result. This assay can detect genomic DNA of H. pylori specifically with as low as 100 pg/reaction by the naked eye. This is a powerful demonstration of G-quadruplex DNAzyme to be used for PCR-based assay with significant advantages of high sensitivity, low cost and simple manipulation over existing approaches and offers the potential opportunity for clinical application.