文献类型：期刊文献

英文题名：Enzyme-activatable fluorescent probes for β-galactosidase: from design to biological applications

作者：Yao, Yongkang[1];Zhang, Yutao[1];Yan, Chenxu[1];Zhu, Wei-Hong[1];Guo, Zhiqian[1]

机构：[1]East China Univ Sci & Technol, Key Lab Adv Mat, Inst Fine Chem,Sch Chem & Mol Engn, Feringa Nobel Prize Scientist Joint Res Ctr,Front, Shanghai 200237, Peoples R China

年份：2021

卷号：12

期号：29

起止页码：9885

外文期刊名：CHEMICAL SCIENCE

收录：;EI(收录号：20213110709441);WOS:【SCI-EXPANDED(收录号:WOS:000674034700001)】；

基金：This work was supported by the National Natural Science Foundation of China (21878087, 21908060), the Innovation Program of Shanghai Municipal Education Commission (2019-01-07-00-02-E00060), Shuguang Program (18SG27), the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Enzymes - Probes - Diseases

摘要：beta-Galactosidase (beta-gal), a typical hydrolytic enzyme, is a vital biomarker for cell senescence and primary ovarian cancers. Developing precise and rapid methods to monitor beta-gal activity is crucial for early cancer diagnoses and biological research. Over the past decade, activatable optical probes have become a powerful tool for real-time tracking and in vivo visualization with high sensitivity and specificity. In this review, we summarize the latest advances in the design of beta-gal-activatable probes via spectral characteristics and responsiveness regulation for biological applications, and particularly focus on the molecular design strategy from turn-on mode to ratiometric mode, from aggregation-caused quenching (ACQ) probes to aggregation-induced emission (AIE)-active probes, from near-infrared-I (NIR-I) imaging to NIR-II imaging, and from one-mode to dual-mode of chemo-fluoro-luminescence sensing beta-gal activity.