文献类型：期刊文献

英文题名：Analysis of the differential metabolic pathway of cultured Chlorococcum humicola with hydroquinone toxic sludge extract

作者：Yang, Yingying[1,2];Zhang, Xinyu[1,2];Hu, Xueyang[1,2];Zhao, Jiamin[1,2];Chen, Xiurong[1,2];Wei, Xiao[1,2];Yu, Xiao[1,2]

机构：[1]East China Univ Sci & Technol, Natl Engn Res Ctr Ind Wastewater Detoxicat & Resou, Sch Resources & Environm Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Environm Protect Key Lab Environm Risk Asses, Sch Resources & Environm Engn, Shanghai 200237, Peoples R China

年份：2022

卷号：370

外文期刊名：JOURNAL OF CLEANER PRODUCTION

收录：;EI(收录号：20223412606732);WOS:【SCI-EXPANDED(收录号:WOS:000861076500009)】；

基金：This research was supported by the National Natural Science Foundation of China (No. 51878278 & 51378207) .

语种：英文

外文关键词：Sludge toxicity; Chlorococcum humicola; protein Interaction; Hydroquinone; Proteomics analysis

摘要：Microbe inhibition produces intermediate metabolites and toxic substances that accumulate significantly in residual sludge toxicity after continuous treatment of coal gasification wastewater with activated sludge. To address this issue, Chlorococcum humicola was cultured with sludge extract. Proteomics analysis was used to investigate the mechanism of stress response in microalgae to sludge toxicity. Upon completion of growth, microalgae treated with sludge extract produced 0.725 +/- 0.036 g/L of biomass. The total organic carbon con-centration and toxicity removal rates on microalgae were 75.1 +/- 3.8% and 47.4 +/- 2.1%, respectively. The proteomics analysis revealed that under stress conditions, microalgae altered cellular components such as the nucleus and plasma membranes, affecting the photosynthetic and respiratory processes. The logarithmic phase was characterized by a down-regulation of oxidative phosphorylation and an increase in energy synthesis regulated by the isopropyl phosphate isomerase. The results demonstrate a proteomic basis for the reduction and utilization of waste sludge, as well as provide a molecular mechanism reference for optimizing the model of cultivating microalgae using sludge.