文献类型：期刊文献

英文题名：Prevalence and genotypic identification of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in pre-weaned dairy calves in Guangdong, China

作者：Feng, Yuanyuan[1];Gong, Xiaoqing[2];Zhu, Kexin[2];Li, Na[2];Yu, Zhengjie[2];Guo, Yaqiong[2];Weng, Yabiao[2];Kvac, Martin[3];Feng, Yaoyu[1,2];Xiao, Lihua[2]

机构：[1]East China Univ Sci & Technol, Sch Resource & Environm, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]South China Agr Univ, Coll Vet Med, Minist Agr, Key Lab Zoonosis, Guangzhou 510642, Guangdong, Peoples R China;[3]Acad Sci Czech Republ, Ctr Biol, Inst Parasitol, Ceske Budejovice, Czech Republic

年份：2019

卷号：12

外文期刊名：PARASITES & VECTORS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000456082800001)】；

基金：This work was supported by the National Natural Science Foundation of China (grant numbers 31820103014 and 31630078).

语种：英文

外文关键词：Cryptosporidium; Giardia duodenalis; Enterocytozoon bieneusi; Molecular epidemiology

摘要：BackgroundCryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi are common enteric pathogens in humans and animals. Data on the transmission of these pathogens are scarce from Guangdong, China, which has a subtropical monsoon climate and is the epicenter for many emerging infectious diseases. This study was conducted to better understand the prevalence and identity of the three pathogens in pre-weaned dairy calves in Guangdong.MethodsThe occurrence and genetic identity of three pathogens were analyzed by polymerase chain reaction. PCR-positive products were sequenced to determine the species and genotypes. A Chi-square test was used to compare the prevalence of pathogens among sampling dates, age groups, or clinical signs.ResultsThe detection rates of Cryptosporidium spp., G. duodenalis and E. bieneusi were 24.0% (93/388), 74.2% (288/388) and 15.7% (61/388), respectively. Three Cryptosporidium species were detected, including C. bovis (n = 73), C. parvum (n = 12) and C. ryanae (n = 7); one animal had concurrence of C. bovis and C. parvum. C. parvum was the dominant species during the first two weeks of life, whereas C. bovis and C. ryanae were mostly seen at 3-9 weeks of age. Sequence analysis identified the C. parvum as subtype IIdA19G1. Assemblage E (n = 282), assemblage A (n = 1), and concurrence of A and E (n = 5) were identified among G. duodenalis-positive animals using multilocus genotyping (MLG). Altogether, 15, 10 and 17 subtypes of assemblage E were observed at the bg, gdh and tpi loci, respectively, forming 49 assemblage E MLGs. The highest detection rate of G. duodenalis was found in winter. Sequence analysis identified genotypes J (n = 57), D (n = 3) and one concurrence of J and D among E. bieneusi-positive animals. The detection rate of E. bieneusi was significantly higher in spring (38.0%; 41/108) than in summer (7.2%; 8/111) and winter (7.1%; 12/169).ConclusionsThese results indicate a common occurrence of C. parvum subtype IIdA19G1, G. duodenalis assemblage E, and E. bieneusi genotype J in pre-weaned dairy calves in Guangdong. More studies are needed to understand the unique genetic characteristics and zoonotic potential of the three enteric pathogens in the province.