文献类型：期刊文献

英文题名：Multifunctional Janus Hematite Silica Nanoparticles: Mimicking Peroxidase-Like Activity and Sensitive Colorimetric Detection of Glucose

作者：Lu, Chang[1,2];Liu, Xiangjiang[2];Li, Yunfeng[3];Yu, Fang[1];Tang, Longhua[1];Hu, Yanjie[3];Yine, Yibin[2]

机构：[1]Zhejiang Univ, State Key Lab Modern Opt Instrumentat, Dept Opt Engn, Hangzhou 310027, Zhejiang, Peoples R China;[2]Zhejiang Univ, Coll Biosyst Engn & Food Sci, Hangzhou 310027, Zhejiang, Peoples R China;[3]E China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Sch Mat Sci & Engn, Shanghai 200237, Peoples R China

年份：2015

卷号：7

期号：28

起止页码：15395

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20153001070659);WOS:【SCI-EXPANDED(收录号:WOS:000358558300033)】；

基金：This work was financially supported by National Natural Science Foundation of China (21305125, 61405176), Zhejiang Educational Committee (Y201225865), Natural Sciences Fund of Zhejiang Province (LY14B050004), and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：hematite-silica; Janus nanoparticle; colorimetric biosensor; hydrogen peroxide; glucose

摘要：The design and engineering of Multifunctional nanostructures with multiple components and synergistic properties are in urgent demand for variety of acceptable biosensing platforms, enabling users to fulfill multiple tasks in a single nanosystem. Herein, we report using an asymmetric hematite silica hybrid of Janus gamma-Fe2O3/SiO2 nanoparticles (JFSNs) as a multifunctional biosensing platform for sensitive colorimetric detection of H2O2 and glucose. It was demonstrated that JFSNs exhibit an intrinsic peroxidase-like catalytic activity. Compared with natural enzyme, JFSNs nanoenzymes could be used over a wider range of pH and temperatures and were more stable over time. Importantly, besides its excellent catalytic activity, the asymmetric properties of the Janus nanoparticle enable it to form the multiple functional utilities for various biosensing applications, including the ease of surface modification without deactivation of catalytic activity and recoverable use by magnetic separation. Thus, we utilized JFSNs with glucose oxidase (GOx) immobilization for glucose-sensitive colorimetric detection, which exhibited both catalytic activity of glucose oxidase and peroxidase with high selectivity and acceptable reproducibility. By combining these two analysis systems into Janus particles, an all-in-one and reusable sensor for blood glucose was formed and has the capability for determination of glucose in,complex samples such as serum. These results suggest that such Janus nanosystems have the potential to construct robust nanoarchitecture with multiple functionalities for various biosensing applications.