文献类型：期刊文献

英文题名：Long-Term Cultivation and Meta-Omics Reveal Methylotrophic Methanogenesis in Hydrocarbon-Impacted Habitats

作者：Liu, Yi-Fan[1,4,5];Chen, Jing[1,5];Liu, Zhong-Lin[1];Hou, Zhao-Wei[2];Liang, Bo[1,5];Wang, Li-Ying[1,5];Zhou, Lei[1,5];Shou, Li-Bin[1,5];Lin, Dan-Dan[1,5];Yang, Shi-Zhong[1,5];Liu, Jin-Feng[1,5];Wu, Xiao-Lin[2];Gu, Ji-Dong[3];Mu, Bo-Zhong[1,5]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Daqing Oilfield Co Ltd, Petrochina, Explorat & Dev Res Inst, Daqing 163712, Peoples R China;[3]Guangdong Technion Israel Inst Technol, Environm Engn & Engn Grp, Shantou 515063, Peoples R China;[4]Shanghai Inst Pollut Control & Ecol Secur, Shanghai 200092, Peoples R China;[5]East China Univ Sci & Technol, Engn Res Ctr Microbial Enhanced Oil Recovery MEOR, Shanghai 200237, Peoples R China

年份：2023

卷号：24

起止页码：264

外文期刊名：ENGINEERING

收录：;EI(收录号：20231814035294);WOS:【SCI-EXPANDED(收录号:WOS:001094710800001)】；

基金：This work was supported by grants of National Natural Science Foundation of China (42061134011, 52074129, and 42173076) ; the Natural Science Foundation of Shanghai (21ZR1417400) ; the Shanghai International Collaboration Program (18230743300) ; the Fundamental Research Funds for the Central Universities (JKJ012016028) to Yi-Fan Liu, Li-Bin Shou, Shi-Zhong Yang, Jin-Feng Liu, and Bo-Zhong Mu; and the NSFC/RGC Joint Research Fund (41161160560) to Ji-Dong Gu.

语种：英文

外文关键词：Methanogenic hydrocarbon degradation; Oily sludge; Bioremediation; Alkanes

摘要：The microbial conversion of alkanes to methane in hydrocarbon-contaminated environments is an intrinsic bioremediation strategy under anoxic conditions. However, the mechanism of microbial methanogenic alkane degradation is currently unclear. Under ten years of continuous efforts, we obtained a methanogenic n-alkane-degrading (C15-C20) enrichment culture that exhibited sustained improvements in the kinetic properties of methane production. The integrated metagenomic and metatranscriptomic analyses revealed that n-alkanes were mainly attacked by members of Desulfosarcinaceae, Firmicutes, and Synergistetes using the fumarate addition strategy, and were then further degraded in a common effort by Tepidiphilus members. Meanwhile, the abundant members of Anaerolineaceae were mainly responsible for cell debris recycling. However, according to the metatranscriptomic analyses, methane was predicted to be produced mainly via H2-dependent methylotrophic methanogenesis, primarily from necromass-derived trimethylamine mediated by Methanomethyliaceae within the candidate phylum Verstraetearchaeota. These findings reveal that H2-dependent methylotrophic methanogens, as well as methylotrophic methanogens, may play important ecological roles in the carbon cycle of hydrocarbon enriched subsurface ecosystems. (c) 2022 THE AUTHORS. Published by Elsevier LTD on behalf of Chinese Academy of Engineering and Higher Education Press Limited Company. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).