文献类型：期刊文献

中文题名：谷胱甘肽的简便测定法

英文题名：A Simple Method for Rapid Determination of Reduced Glutathione

作者：赵旭东[1];魏东芝[1];万群[1];俞俊棠[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2000

卷号：20

期号：1

起止页码：34

中文期刊名：药物分析杂志

外文期刊名：Chinese Journal of Pharmaceutical Analysis

收录：CSTPCD;;北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：谷胱甘肽;甲醛;关胱氨酸;DTNB

外文关键词：glutathione, formaldehyde, cysteine, DTNB

摘要：目的： 建立一种快速简便测定谷胱甘肽的新方法。方法： 样品１ 式２ 份， ｐＨ８－０ 条件下分别和甲醛反应２ｍｉｎ 和６０ ｍｉｎ， 各取１ ｍＬ加入５ ｍＬＤＴＮＢ溶液， ２５ ℃反应５ ｍｉｎ 后分别测定在波长４１２ ｎｍ 的吸光度， 算出２者的差值ΔＡ， 代入标准曲线计算得出谷胱甘肽含量。结果： 谷胱甘肽浓度在０－１９ ～０－９５ ｇ·Ｌ－１ 之间线性关系良好， 回归方程为： Ｙ＝ ０－７１５ ４ Ｘ－ ０－０００ ４， ｒ＝ ０－９９９ ９ ， 最大误差不超过６ ％ 。结论： 方法成本低廉， 简单易行， 特别适合于有干扰物质存在时还原型谷胱甘肽浓度的分析。
Objective:A new simple method for rapid determination of reduced glutathione in the presence of other thiols is developed based on the reactions of formaldehyde with glutathione and interefering substances. Method:After each aliquot of two same samples reacts with formaldehyde for 2 and 60 min respectively, 1 mL of each solution is added to 5 mL DTNB and reacts for 5 min at 25 ℃. The absorbance is measured immediately at wavelength 412 nm. The difference between two absorbance(Δ A = A 2 min - A 60 min )is calculated, concentration of glutathione can be obtained from standard curve. Result:There is a good linearity between 0.19 to 0 95 g·L -1 glutathione. Regression equation: Y=0 715 4X-0 000 4,r=0 999 9, maximum deviation is less than 6%. Conclusion:The method is cheap, simple, practicable and is applicable to assay of reduced glutathione in the presence of other interefering substances.