文献类型：期刊文献

英文题名：A functional drug delivery platform for targeting and imaging cancer cells based on Pluronic F127

作者：Zhang, Denghao[1,2];Tao, Liang[1,2];Zhao, Hongli[1,2];Yuan, Huihui[1,2];Lan, Minbo[1,2,3]

机构：[1]E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Res Ctr Anal & Test, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2015

卷号：26

期号：8

起止页码：468

外文期刊名：JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION

收录：;EI(收录号：20151600749181);WOS:【SCI-EXPANDED(收录号:WOS:000353479400002)】；

基金：This work was supported by the Science and Technology Commission of Shanghai Municipality (STCSM, contract Nos. 11nm0505700 and 13142201001).

语种：英文

外文关键词：fluorescent imaging; targeted delivery; solasodine; functional micelles; Pluronic F127

摘要：Functional polymeric micelles play an important role in the efficient delivery of therapeutic drugs into tumours. In this study, a functional drug delivery platform with ligands for targeting and fluorescent imaging was designed based on Pluronic F127 (PF127). Using folic acid (FA) and fluorescein isothiocyanate (FITC) to chemically conjugate with PF127, two functional polymers, Pluronic F127-FA (PF127-FA) and Pluronic F127-FITC (PF127-FITC), were synthesized. Solasodine-loaded micelles were then prepared via the thin-film hydration method. By employing A549 and HeLa cells, the results of in vitro cell assays performed using confocal laser scanning microscopy and flow cytometry suggested that the proposed micelles could provide the desired specific targeting and fluorescent imaging functions. In addition, the results of in vitro cytotoxicity experiments showed that the growth inhibition rates of A549 and HeLa cells treated with solasodine-loaded micelles were remarkably higher than those of cells treated with free solasodine. Solasodine-loaded micelles exhibited a more distinct inhibitory effect against HeLa cells than against A549 cells. Thus, an effective drug delivery system for targeting and imaging cancer cells was developed.