文献类型：期刊文献

英文题名：Self-Assembled Injectable Nanocomposite Hydrogels Coordinated by in Situ Generated CaP Nanoparticles for Bone Regeneration

作者：Kuang, Lijun[1,2];Ma, Xiaoyu[1,2];Ma, Yifan[1,2];Yao, Yuan[2];Tariq, Muhammad[1];Yuan, Yuan[1,2,3];Liu, Changsheng[1,2,3]

机构：[1]East China Univ Sci & Technol, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Mat Sci & Engn, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Minist Educ, Engn Res Ctr Biomat, Shanghai 200237, Peoples R China

年份：2019

卷号：11

期号：19

起止页码：17234

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20192106965547);WOS:【SCI-EXPANDED(收录号:WOS:000468364500013)】；

基金：The authors wish to express their gratitude to the National Natural Science Foundation of China for Innovative Research Groups (No. 51621002), National Key R&D Program of China (2018YFC1105700), the National Natural Science Foundation of China (No. 31771040), and Leading Talents in Shanghai in 2017 for financial support.

语种：英文

外文关键词：in situ generated CaP nanoparticles; injectable nanocomposite hydrogels; self-assembly; Apt-functionalized; bone regeneration

摘要：Due to the great similarity to the natural extracellular matrix and minimally invasive surgeries, injectable hydrogels are appealing biomaterials in cartilage and bone tissue engineering. Nevertheless, undesirable mechanical properties and bioactivity greatly hamper their availability in clinic applications. Here, we developed an injectable nano-composite hydrogel by in situ growth of CaP nanoparticles (ICPNs) during the free-radical polymerization of dimethy-laminoethyl methacrylate (DMAEMA) and 2-hydroxyethyl methacrylate (HEMA) matrix (PDH) for bone regeneration. The ICPNs are self-assembled by incorporation of poly-L-glutamic acid (PGA) with abundant carboxyl functional groups during the formation of carboxyl-Ca2+ coordination and further CaP precipitation. Furthermore, the carboxyl groups of PGA could interact with the tertiary amines of DMAEMA fragments and thus improve the mechanical strength of hydrogels. Upon mixing solutions of DMAEMA and HEMA bearing PGA, Ca2+, and Po-4(3-), this effective and dynamic coordination led to the rapid self-assembly of CaP NPs and PDH nanocomposite hydrogels (PDH/mICPN). The obtained optimal nanocomposite hydrogels exhibited suitable injectable time, an enhanced tensile strength of 321.1 kPa, and a fracture energy of 29.0 kJ/m(2) and dramatically facilitated cell adhesion and upregulated osteodifferentiation compared to hydrogels prepared by blending ex situ prefabricated CaP NPs. In vivo experiments confirmed the promoted osteogenesis, which shows a striking contrast to pure PDH hydrogels. Additionally, the methacrylate groups on the monomers could easily be functionalized with aptamers and thereby facilitate recognition and capturing of bone marrow stromal cells both in vitro and in vivo and strengthen the bone regeneration. We believe that our conducted research about in situ self-assembled CaP nanoparticle-coordinated hydrogels will open a new avenue for bone regeneration in the future endeavors.