文献类型：期刊文献

英文题名：Cellulase mediated stress triggers the mutations of oleaginous yeast Trichosporon cutaneum with super-large spindle morphology and high lipid accumulation

作者：Liu, Qi[1];Li, Yuanyuan[1];Hou, Weiliang[1];Zhang, Bin[1,2];Bao, Jie[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2023

卷号：18

期号：8

外文期刊名：BIOTECHNOLOGY JOURNAL

收录：;EI(收录号：20232114133292);WOS:【SCI-EXPANDED(收录号:WOS:000990753800001)】；

基金：ACKNOWLEDGMENTS This research was supported by the National Natural Science Foundation of China (21978083); the Yangfan Project of Science and Technology Committee of Shanghai Municipality (23YF1409900).

语种：英文

外文关键词：cell size; cell wall structure; cellulase; microbial lipid; oleaginous yeast

摘要：Accumulation of intracellular lipid bodies in oleaginous yeast cells is highly restricted by their natural intracellular space. Here we show a cellulase mediated adaptive evolution with ultra-centrifugation fractionation of oleaginous yeast Trichosporon cutaneum to obtain the favorable cell structure for lipid accumulation. Cellulase was added to the wheat straw hydrolysate during long-term adaptive evolution for disruption of cell wall integrity of T. cutaneum cells. The cellulase, together with ultracentrifugation force, triggered multiple mutations and transcriptional expression changes of the functional genes associated with cell wall integrity and lipid synthesis metabolism. The fractionated mutant T. cutaneum YY52 demonstrated the heavily weakened cell wall and high lipid accumulation by the super-large expanded spindle cells (two orders of magnitude greater than the parental). A record-high lipid production by T. cutaneum YY52 was achieved (55.4 +/- 0.5 g L-1 from wheat straw and 58.4 +/- 0.1 g L-1 from corn stover). This study not only obtained an oleaginous yeast strain with industrial application potential for lipid production but also provided a new method for generation of mutant cells with high intracellular metabolite accumulation.