文献类型：期刊文献

中文题名：以超临界CO_2为稀释剂的胆红素重结晶提纯

英文题名：Refining of Bilirubin with Supercritical Carbon Dioxide Antisolvent Recrystallization

作者：蔡建国[1];杨中文[1];周展云[1]

机构：[1]华东理工大学化学工程研究所

年份：1994

卷号：20

期号：3

起止页码：317

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;国家哲学社会科学学术期刊数据库;Scopus;CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：超临界流体;CO2;稀释剂;胆红素;重结晶;提纯

外文关键词：bilirubin;supercritical fluid;carbon dioxide;refining;recrystallization

摘要：以二甲基亚砜（ＤＭＳＯ）为溶剂，以超临界ＣＯ＿２为稀释膨胀剂，重结晶提纯胆红素。考察了ＤＭＳＯ膨胀度对胆红素溶解能力的影响。结果表明，胆红素的ＤＭＳＯ溶液经超临界ＣＯ＿２稀释膨胀至３倍后，ＤＭＳＯ对胆红素的溶解能力显著下降。用不同的温度、压力、溶液加入量等条件，能有效地控制ＤＭＳＯ的稀释膨胀程度，从而在短时间内形成较大的过饱和度，使胆红素析出，分离母液后，获得长度＜１μｍ、直径＜０．５μｍ和颗粒较均匀的胆红素，纯度大于９０％。
The process of recrystallization with supercritical carbon dioxide as an anti-solvent agent was studied.The expansion of Dimethyl Sulphoxide(DMSO)influencing the solubility of Bilirubin was discussed.The results showed:In different temperature,pressure and different amount of solvent can form different supersaturation ratio.When the DMSO solution of bilirubin is expanded and then the solvent is extracted, it can obtain a particle less than lum with bilirubin content more than ninety percent.It appears to be a reliable and effeclive method for refining of biologically active products and micronizing.