文献类型：期刊文献

英文题名：Characterization of a TetR-type positive regulator AtrA for lincomycin production in Streptomyces lincolnensis

作者：Wu, Wei[1,2];Kang, Yajing[1,2];Hou, Bingbing[1,2];Ye, Jiang[1,2];Wang, Ruida[1,2];Wu, Haizhen[1,2];Zhang, Huizhan[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Dept Appl Biol, Shanghai, Peoples R China

年份：2023

卷号：87

期号：7

起止页码：786

外文期刊名：BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

收录：;EI(收录号：20233014444491);WOS:【SCI-EXPANDED(收录号:WOS:000980130800001)】；

基金：This work was supported by the National Key Research and Development Program of China (2021YFC2100600) and the National Natural Science Foundation of China (NSFC) ( 31900059 ) .

语种：英文

外文关键词：Streptomyces lincolnensis; AtrA; lincomycin production; regulatory pathway

摘要：AtrA belongs to the TetR family and has been well characterized for its roles in antibiotic biosynthesis regulation. Here, we identified an AtrA homolog (AtrA-lin) in Streptomyces lincolnensis. Disruption of atrA-lin resulted in reduced lincomycin production, whereas the complement restored the lincomycin production level to that of the wild-type. In addition, atrA-lin disruption did not affect cell growth and morphological differentiation. Furthermore, atrA-lin disruption hindered the transcription of regulatory gene lmbU, structural genes lmbA and lmbW inside the lincomycin biosynthesis gene cluster, and 2 other regulatory genes, adpA and bldA. Completement of atrA-lin restored the transcription of these genes to varying degrees. Notably, we found that AtrA-lin directly binds to the promoter region of lmbU. Collectively, AtrA-lin positively modulated lincomycin production via both pathway-specific and global regulators. This study offers further insights into the functional diversity of AtrA homologs and the mechanism of lincomycin biosynthesis regulation.