文献类型：期刊文献

英文题名：Anaphthalimide-based fluorescent probe for mercapto-containing compounds

作者：Song, Lun[1];Sun, Xu-Dong[1];Ge, Yu[2];Yao, Yu-Hua[1];Shen, Jie[1];Zhang, Wei-Bing[1];Qian, Jun-Hong[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Shanghai Inst Qual Inspect & Tech, Shanghai 200233, Peoples R China

年份：2016

卷号：27

期号：12

起止页码：1776

外文期刊名：CHINESE CHEMICAL LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000390724500010)】；

基金：This work was financially supported by NSFC (Nos. 21576085 and 21475044), Shanghai Municipal Natural Science Foundation (No. 15ZR1409000).

语种：英文

外文关键词：Polarity; Sulfur-containing proteins; Thiol; Fluorescent probe; Naphthalimide

摘要：A polarity-sensitive fluorescent probe MNP was rationally designed and synthesized with naphthalimide as the fluorophore and maleimide as the receptor for thiols. MNP is weakly fluorescent due to the photoinduced electron-transfer (PET) from the fluorophore to the receptor, and it displays evidently solvatochromic UV-vis and fluorescence spectra: the emission shifted from 495 nm in n-hexane to 545 nm in phosphate buffer solution. Michael addition reaction between thiols and the maleimide in MNP inhibited the PET process, which led to about eight-fold fluorescence enhancement. In addition, MNP showed highly sensitivity to mercapto-containing proteins and it could detect as low as 20.4 mu g/mL of BSA in PBS. MNP has potential in fluorescent imaging of thiols in living cells. (C) 2016 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.