文献类型：期刊文献

英文题名：Dual-pH-sensitivity and tumour targeting core-shell particles for intracellular drug delivery

作者：Hao, Weiju[1];Shen, Yinxing[1];Liu, Danyang[1];Shang, Yazhuo[1];Zhang, Junqi[2,3];Xu, Shouhong[1];Liu, Honglai[1]

机构：[1]East China Univ Sci & Technol, Coll Chem & Mol Engn, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]Fudan Univ, Minist Hlth, Key Lab Med Mol Virol, 138 Yixueyuan Rd, Shanghai 200032, Peoples R China;[3]Fudan Univ, Minist Educ, Sch Basic Med Sci, 138 Yixueyuan Rd, Shanghai 200032, Peoples R China

年份：2017

卷号：7

期号：2

起止页码：851

外文期刊名：RSC ADVANCES

收录：;EI(收录号：20170203239253);WOS:【SCI-EXPANDED(收录号:WOS:000393743000034)】；

基金：Financial support for this study is provided by the National Natural Science Foundation of China (No. 21276074), the 111 Project (No. B08021) of China and the Fundamental Research Funds for the Centre Universities of China.

语种：英文

外文关键词：Shells (structures) - Biocompatibility - Cell death - Tumors - pH sensors - Controlled drug delivery - Core shell nanoparticles - Targeted drug delivery - Silica nanoparticles

摘要：The principal problem in the area of drug delivery is achieving better selectivity and controllability. A new core-shell nanoparticle composite (denoted MSN@Tf@Polymer) with dual-pH-sensitivity has been prepared as a drug carrier for intracellular drug delivery and release. MSN@Tf@Polymer consists of mesoporous silica nanoparticles (MSN), green-transferrin (Tf) and diblock copolymer (poly-2di-isopropylamino ethylmethacrylate-b-methoxy-poly ethyleneglycol: mPEG45-PDPAn). The core-shell structure is self-assembled layer by layer. Results show that nearly 80% doxorubicin hydrochloride (DOX) loaded in MSN@Tf@Polymer could be released in 5 h at pH 5.0, which is an improvement from the results obtained at pH 6.5 and pH 7.4. MTT assay and fluorescence inversion microscope experiments indicate that MSN@ Tf is successfully taken up by liver cancer cells (Huh7) without apparent cytotoxicity, and Tf has strong intensity of fluorescence for subcellular localization. Confocal laser scanning microscopy (CLSM) experiments indicate that MSN@Tf@Polymer is able to enter the lysosome of the tumor cells. Furthermore, cell apoptosis experiments prove that DOX loaded in MSN@Tf@Polymer has the best anti-tumor effect compared with free DOX and DOX in bare MSN. MSN@Tf@Polymer has high biocompatibility, enhanced drug loading, site-specific delivery and in situ stimulus release and will also hopefully be applied as an intracellular drug delivery system.