文献类型：期刊文献

英文题名：Integrated surface display and atp regeneration for efficient butyrolactam biosynthesis in Escherichia coli

作者：Cui, Xiangcao[1];Liu, Xiaosen[1];Zhang, Huiling[1];Zhou, Kun[1];Tang, Cunzhuo[1];Deng, Chen[1,2];Li, Xu[1,2];Qiu, Yongjun[1,2];Fan, Liqiang[1,2];Zhao, Liming[1,2]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China

年份：2026

卷号：235

外文期刊名：BIOCHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20262721057141);Scopus(收录号：2-s2.0-105043789599);WOS:【SCI-EXPANDED(收录号:WOS:001820737600001)】；

基金：This work was financially supported by the 2024 Shanghai Action Plan for Science, Technology and Innovation. (No. 24HC2820700) , and Fundamental and Interdisciplinary Disciplines Breakthrough Plan of the Ministry of Education of China.

语种：英文

外文关键词：Butyrolactam; Surface display; beta-alanine CoA transferase; ATP regeneration; Biocatalysis

摘要：Butyrolactam is a high value-added monomer and an important intermediate for various physiologically active substances, and has been widely used in the pharmaceutical, pesticide, and chemical industries. Though the biosynthesis of butyrolactam is gradually advancing, challenges such as the requirement for expensive cofactor adenosine triphosphate (ATP) and the barrier of substrate transmembrane transport in whole-cell synthesis hinder its large-scale industrial application. In this study, an integrated whole-cell catalytic strategy combining ice nucleation protein (INP)-mediated surface display and ATP regeneration was developed. Compared with the butyrolactam productivity of cells intracellularly expressing beta-alanine CoA transferase (ACT), co-expressing polyphosphate kinase 2 (PPK2) to regenerate ATP and display ACT on the cell surface only increased it by 23.1% and 25.0%, respectively. Co-displaying ACT and PPK2 further increased the butyrolactam productivity, which was 46.4% more than that of only ACT displayed cells. Using fed-batch fermentation strategy, a final butyrolactam titer of 39.13 g/L and a yield of 94.7% were achieved in the strain co-displaying ACT and PPK2. Our findings demonstrate a potentially attractive strategy for the industrial butyrolactam production.