文献类型：期刊文献

英文题名：Salinity-Aided Selection of Progressive Onset Denitrifiers as a Means of Providing Nitrite for Anammox

作者：Li, Wei[1,2,3,4];Li, Hui[1];Liu, Yong-di[1];Zheng, Ping[2];Shapleigh, James P.[3]

机构：[1]East China Univ Sci & Technol, Sch Resources & Environm Engn, Natl Engn Lab Ind Wastewater Treatment, Shanghai 200237, Peoples R China;[2]Zhejiang Univ, Coll Environm & Resource Sci, Dept Environm Engn, Hangzhou 310027, Zhejiang, Peoples R China;[3]Cornell Univ, Dept Microbiol, Ithaca, NY 14850 USA;[4]Shanghai Inst Pollut Control & Ecol Secur, Shanghai 200092, Peoples R China

年份：2018

卷号：52

期号：18

起止页码：10665

外文期刊名：ENVIRONMENTAL SCIENCE & TECHNOLOGY

收录：;EI(收录号：20183805832298);WOS:【SCI-EXPANDED(收录号:WOS:000445441000048)】；

基金：This work was supported by National Natural Science Foundation of China (51578240), Fundamental Research Funds for the Central Universities (222201814053, 222201817009), and Research Fund Program of Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology (2018K16). The authors would like to thank Natalie Morse for reading an earlier version of the manuscript.

语种：英文

外文关键词：Denitrification - Mapping - Ammonia - Efficiency - Genes - Nitrogen removal - Wastewater treatment

摘要：Anaerobic ammonium oxidation (anammox) combined with partial-denitrification (NO3- -> NO2-) is an innovative process for the simultaneous removal of ammonia and nitrate from wastewaters. An efficient method for the selection of partial denitrifying community, which relies on increasing influent salinity, is described. Using this method, a denitratating community was enriched, which showed a nitrite accumulation efficiency higher than 75% as well as a high nitrate conversion efficiency. Community analysis using 16S rDNA indicated that Halomonas became the dominant genus as salinity increased. Metagenomic analysis revealed that there was not a significant difference in reads mapping to downstream denitrification genes in a comparison of samples from cultures with 5% salinity to those without salinity. The majority of the reads mapping to the genes encoding dissimilatory nitrate and nitrite reductases nar and nirS came from Halomonas under high salinity conditions. Two metagenome-assembled genomes taxonomically assigned to Halomonas were obtained, one of which accounted for similar to 35% of the reads under high salinity conditions. Both genomes harbored the genes for the complete denitrification pathway. These results indicate progressive onset denitrifiers, a phenotype where nitrite reduction only occurs after nitrate exhaustion, could be successfully enriched with increasing salinity. Progressive onset denitrifiers may be more widespread in natural and artificial habitats than anticipated and are shown here to be valuable for nitrogen mitigating processes.