文献类型：期刊文献

英文题名：Detection of tumor immunity-related ENPP1 using AIE-active probe QM-AMP

作者：Wang, Yuwei[1,2];Yang, Ting[1,2];Liu, Shichang[1,2];Yang, Haojian[1,2];Xu, Qin[3];Zhu, Wei-Hong[1,2];Shao, Andong[3];Wang, Qi[1,2]

机构：[1]East China Univ Sci & Technol, Inst Fine Chem, Sch Chem & Mol Engn, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Inst Fine Chem,Joint Int Res Lab Precis Chem & Mol, Sch Chem & Mol Engn,Shanghai Key Lab Funct Mat Che, Shanghai 200237, Peoples R China;[3]Jiangnan Univ, Sch Life Sci & Hlth Engn, Key Lab Carbohydrate Vaccines & Drugs Jiangsu Prov, Wuxi 214122, Peoples R China

年份：2026

卷号：7

期号：4

起止页码：363

外文期刊名：GREEN CHEMICAL ENGINEERING

收录：WOS:【ESCI(收录号:WOS:001803217000001)】；

基金：This work was supported by the National Key Research and Development Program of China (2024YFA1803501), the NSFC Excellent Young Scientist (EYS) Scheme (22222803), the Basic Science Center of NSFC (T2488302), and the Science and Technology Commission of Shanghai Municipality (24DX1400200).

语种：英文

外文关键词：ENPP1; Aggregation-induced emission; Amphiphilic fluorescent probe; Macrophage polarization

摘要：Ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), a crucial biomarker on the surface of tumor cells, plays a significant role in immune suppression and cancer metastasis. In this study, we successfully developed a novel amphiphilic fluorescent probe, QM-AMP, based on quinoline-malononitrile aggregation-induced emission (AIE) characteristics, for the real-time, and specific detection of ENPP1 enzymatic activity in multiple cell types. QM-AMP exhibits excellent dispersibility in both hydrophilic and hydrophobic environments, effectively minimizing background fluorescence interference and ensuring high sensitivity in ENPP1 detection. Upon interaction with ENPP1, the AMP group of the probe is specifically recognized and cleaved by ENPP1, which triggers the cleavage of the O-P bond and results in an "off-on" AIE fluorescence signal. QM-AMP demonstrates favorable photostability and biocompatibility, showing clear differential responses across cells with varying ENPP1 expression levels. Additionally, it can monitor ENPP1 expression changes under different macrophage polarization states, providing a potential tool for investigating ENPP1's role in the tumor immune microenvironment. This study not only offers a new approach for in-situ detection of ENPP1 as a biomarker but also opens up new possibilities for early diagnosis and treatment of related diseases.