文献类型：期刊文献

英文题名：A cytosensor based on NiO nanoparticle-enhanced surface plasmon resonance for detection of the breast cancer cell line MCF-7

作者：Jia, Shengsong[1];Li, Pengfei[2];Koh, Kwangnak[3];Chen, Hongxia[1]

机构：[1]Shanghai Univ, Sch Life Sci, Lab Biosensing Technol, Shanghai 200444, Peoples R China;[2]E China Univ Sci & Technol, Sch Biotechnol, Shanghai 200237, Peoples R China;[3]Pusan Natl Univ, Inst Gen Educ, Busan 609735, South Korea

年份：2016

卷号：183

期号：2

起止页码：683

外文期刊名：MICROCHIMICA ACTA

收录：;EI(收录号：20242616459958);WOS:【SCI-EXPANDED(收录号:WOS:000370176500021)】；

基金：This work is supported by the National Natural Science Foundation of China (Grant Nos. 61275085, 31100560).

语种：英文

外文关键词：Aptamer; His tag; MUC-1; Diagnosis; Tumor marker; Islet beta cells; Biosensor

摘要：The article describes the use of a designed histidine (His)-tagged arginine-glycine-aspartic acid (RGD) peptide as a linker to bind integrin to captured cancer cells, and how to amplify the surface plasmon resonance (SPR) signal after binding of NiO nanoparticles (NiO-NPs) via the His-tag on the peptide. Specifically, breast cancer cells were captured via the interaction between human mucin (MUC-1) and a gold surface modified with a MUC-selective aptamer. The resulting cytosensor exhibits specificity and high sensitivity which is due to the enhancement of the SPR signal by NiO-NPs. The breast cancer cell line MCF-7 can be easily distinguished from normal islet beta cells by using this biosensor. Implementation of the His-tagged RGD peptide modified with NiO-NPs resulted in 20-fold enhancement of the SPR signal at the limit of detection. Hence, the actual limit of detection is lowered to 136 cells per mL. In our perception, this cytosensor has a large clinical potential in that it may also be used to detect various other kinds of tumor cells.