文献类型：期刊文献

英文题名：Dual-Emitting Carbonized Polymer Dots Synthesized at Room Temperature for Ratiometric Fluorescence Sensing of Vitamin B12

作者：Gao, Ya-Ting[1];Chen, Bin-Bin[1];Jiang, Lei[1];Lv, Jian[1];Chang, Shuai[1];Wang, Yue[1];Qian, Ruo-Can[1];Li, Da-Wei[1];Hafez, Mahmoud Elsayed[1,2]

机构：[1]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]Beni Suef Univ, Fac Sci, Dept Chem, Bani Suwayf 62511, Egypt

年份：2021

卷号：13

期号：42

起止页码：50228

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20214411100219);WOS:【SCI-EXPANDED(收录号:WOS:000713052700061)】；

基金：The authors appreciate financial support from the National Natural Science Foundation of China (21788102, 21777041, 21974046, 22176058, and 21977031), the Science and Technology Commission of Shanghai Municipality (19391901700, 19520744000, and 19ZR1472300), and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：dual-emitting carbonized polymer dots; room-temperature synthesis; fluorescent ratiometry; vitamin B12 detection

摘要：Ratiometric fluorescence (FL) probes are highly desirable for highly sensitive and reliable assays. Dual-emitting carbonized polymer dots (CPDs) have great application prospects in building ratiometric FL sensors. However, dual-emitting CPDs are usually synthesized at high temperatures and high pressures, which not only increases the cost but also complicates the structure of CPDs. Here, we developed a facile strategy for the fabrication of dual-emitting CPDs at room temperature using tetrachlorobenzoquinone and ethylenediamine. The formation of CPDs was induced by Schiff base condensation reaction, enabling the following cross-linking polymerization process. The dual-emitting CPDs demonstrate good photostability and antioxidant capacity. Importantly, the typical dual-emission bands of the as-prepared CPDs are found to have a blue emission band at 445 nm with a maximum excitation of 350 nm and a yellow emission band at 575 nm with a maximum excitation of 440 nm. Based on the dual-emitting property of CPDs, a ratiometric FL nanoprobe is obtained for sensitive determination of vitamin B12 (VB12), as the inner filtering and static quenching effects between VB12 and CPDs allow effective quenching of the blue FL of CPDs, while the yellow FL is maintained. The established assay shows linear detection ranges of 0.25-100 mu M with a low limit of detection of 0.14 mu M. These findings provide new guidance for the facile preparation of CPDs with excellent dual-emitting optical properties, indicating good prospects in biosensing.