文献类型：期刊文献

英文题名：High-throughput screening strategy used for enhanced production of pigment by Monascus purpureus D39-4

作者：Tan, Jun[1];Chu, Ju[1];Shi, Wenjuan[1];Lin, Cheng[1];Guo, Yuanxin[1];Zhuang, Yingping[1];Zhang, Siliang[1];Imanaka, Tadayuki[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2012

卷号：21

期号：6

起止页码：1603

外文期刊名：FOOD SCIENCE AND BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000313078600013)】；

基金：This work was financially supported by a grant from the National High Technology Research and Development Program of China (863 Program) 2012AA021201, and the Major State Basic Research Development Program of China (973 Program), No. 2012CB721006 and National Major Scientific and Technological Special Project, No. 2012YQ15008709.

语种：英文

外文关键词：Monascus; Box-Behnken design; response surface methodology; medium optimization; high-throughput screening

摘要：Most of the fermentation experiment designs were limited by the low-throughput of shake flask, especially for the medium optimization. A simple high-throughput screening system was developed for the determination of pigment in Monascus purpureus fermentation samples. This downscaled system was designed to optimize medium composition combined with statistical methods. The total 29 experiments designed by the Box-Behnken were used to study the 4 most important operating variables on pigment production. The analysis revealed that the optimum concentrations of glucose, peptone, NaNO3, and KH2PO4 were 51.42, 4.91, 1.00, and 1.00 g/L, respectively. A production of 69.5 U/mL was achieved in agreement with the prediction (68.9 U/mL) fermented in 24-deep-well microtiterplates. Furthermore, the fermentation medium optimized in the high-throughput system was verified in shake flasks, and the pigment production could be enhanced from 206.5 U/mL in un-optimized medium to 265.8 U/mL, giving nearly 1.30-fold increase in production.