文献类型：期刊文献

中文题名：茯苓多糖HPLC指纹图谱与免疫活性的相关分析

英文题名：Correlation Analysis between HPLC Fingerprint of Polysaccharides from Poria Cocos and Immunological Activity

作者：肖颖[1];吴梦琪[1];张文清[1];徐志珍[1];夏玮[1]

机构：[1]华东理工大学化学与分子工程学院,上海市功能性材料化学重点实验室,上海200237

年份：2020

卷号：46

期号：5

起止页码：672

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD_E2019_2020】；

语种：中文

中文关键词：茯苓多糖;单糖组成;HPLC;指纹图谱;免疫活性;正交偏最小二乘分析

外文关键词：polysaccharides of Poria cocos;monosaccharide composition;HPLC;fingerprint;immunological activity;orthogonal partial least squares analysis

摘要：通过高效液相色谱(HPLC)图建立16批不同产地茯苓多糖的指纹图谱,以NO释放浓度为免疫活性指标,测定16批茯苓多糖刺激处于对数生长期的小鼠巨噬细胞产生的NO的浓度,并利用正交偏最小二乘分析,研究茯苓多糖单糖组成与免疫活性间的相关性。结果表明:茯苓多糖由葡萄糖、半乳糖、甘露糖、岩藻糖、氨基葡萄糖、葡萄糖醛酸、木糖以及阿拉伯糖等8种单糖组成;用不同浓度茯苓多糖刺激小鼠巨噬细胞,NO释放浓度随着茯苓多糖浓度的增大而增大,且均高于空白对照组;茯苓多糖中各单糖对小鼠巨噬细胞释放NO的浓度均有相关性,半乳糖、阿拉伯糖、甘露糖及葡萄糖对小鼠巨噬细胞的免疫活性呈正相关,其中具有显著性贡献的是葡萄糖、半乳糖和甘露糖。
Poria cocos,called FuLing in Chinese,is a traditional Chinese medicinal herb.Poria cocos polysaccharides(PCPs)were extracted from Poria cocos collected from different origins through water extraction and alcohol precipitation.High performance liquid chromatography(HPLC)was applied to establish the fingerprint for 16 batches PCPs.A macrophage cell line RAW274.7 was incubated in different concentrations of 16 batches PCPs.The immunological activity was evaluated based on the proliferation activity of cells as determined by MTT assay and NO production.The immunological activity of 16 batches PCPs were introduced into orthogonal partial least squares analysis to investigate the correlation between monosaccharide compositions and immunological activity.The results showed that PCPs were composed of eight monosaccharides,which were Glc,Gal,Man,Fuc,GlcN,GlcA,Xyl and Ara,respectively.The similarity analysis showed that the similarity of the 16 batches PCPs ranged from 0.980 to 1.000.All the 16 PCPs showed well immunological activities,according to the in vitro activity evaluations.Mouse macrophages were stimulated with different concentrations of PCPs.NO production increased with the increase of PCPs concentration,which was higher than that of the blank.According to the orthogonal partial least squares analysis,all the monosaccharides from PCPs had relationship with immunological activity.Gal,Ara,Man and Glc were positively correlated with the immunological activity of mouse macrophages,while Fuc,GlcA,GlcN and Xyl were negative correlated with the immunological activity of mouse macrophages.Particularly,Glc,Gal and Man in PCPs significantly contributed to the NO production from mouse macrophages.