文献类型：期刊文献

中文题名：Feiji Recipe inhibits the growth of lung cancer by modulating T-cell immunity through indoleamine-2,3-dioxygenase pathway in an orthotopic implantation model

英文题名：Feiji Recipe inhibits the growth of lung cancer by modulating T-cell immunity through indoleamine-2,3-dioxygenase pathway in an orthotopic implantation model

作者：Bin Luo[1];Zu-jun Que[2];Zhi-yi Zhou[1];Qing Wang[2];Chang-sheng Dong[2];Yi Jiang[1];Bing Hu[1,2];Hui Shi[3];Yu Jin[3];Jian-wen Liu[3];He-gen Li[1];Lin Wang[4];Jian-hui Tian[1,2]

机构：[1]Department of Oncology, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine;[2]Oncology Institute of Traditional Chinese Medicine, Institute of TCM Oncology;[3]School of Pharmacy, East China University of Science and Technology;[4]Department of Nephrology, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

年份：2018

卷号：16

期号：4

起止页码：283

中文期刊名：Journal of Integrative Medicine

外文期刊名：结合医学学报（英文版）

收录：CSTPCD;;Scopus;CSCD:【CSCD2017_2018】；PubMed;

基金：supported by National Natural Science Foundation of China(No.81173224 and 81373621);Shanghai Municipal Committee of Science and Technology(No.11ZR1437000);the Science and Technology Commission of Shanghai Municipality(No.12401905700);Longhua Medical Project(D-11)(all to Jian-hui Tian);State Administration of Traditional Chinese Medicine Clinical Research Base Special Project(No.JDZX2012125)(He-gen Li);Action Plan of Shanghai Chinese Medicine for Three Years(No.ZY3-CCCX-2-1002)(Zhen Xiao)

语种：英文

中文关键词：Feiji Recipe Immune escape Orthotopic xenografts;Indoleamine-2,3-dioxygenase

外文关键词：Feiji Recipe Immune escape Orthotopic xenografts;Indoleamine-2,3-dioxygenase

摘要：Objective： Escape from the body＇s immune response is a basic characteristic of lung cancer, and indoleamine-2,3-dioxygenase （IDO） plays a key role in mediating immune escape of non-small-cell lung cancer, which leads to recurrence and metastasis. Feiji Recipe, a compound Chinese herbal medicine, has the effect of stabilizing lesions and prolonging survival in patients with lung cancer. The purpose of this study was to investigate the mechanisms underlying the anticancer properties of Feiji Recipe. Methods： An orthotopic transplant model of mouse Lewis lung cancer, with stable expression of IDO gene, was established in C57BL/6 mice. Optical imaging was used to observe the effects of Feiji Recipe in the treatment of lung cancer in vivo. The effects of Feiji Recipe on the proliferation of mouse Lewis lung cancer cell line 2LL, 2LL-enhanced green fluorescent protein （2LL-EGFP） and 2LL-EGFP-IDO were investi- gated, and the apoptosis of T-cells was examined by 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2H-tetra zolium bromide using flow cytometry. Chemical composition of Feiji Recipe was validated by high- performance liquid chromatography. Results： Compared to the control group, the survival of animals treated with Feiji Recipe was significantly prolonged （P〈0.0074）, and the IDO protein level decreased （P=0.0072）; moreover, the percentages of CD4＋CD25＋ T-cells and Foxp3＋ T-cells were significantly decreased （P〈0.05）. The molecular mechanism of Feiji Recipe against lung cancer may relate to the regulation of immune cells, such as T-cells and reg- ulatory T-cells. Conclusion： The molecular mechanism of Feiji Recipe in treatment of lung cancer is to restore the function of T-cells in the cancer microenvironment through interfering with the IDO pathway.
Objective： Escape from the body＇s immune response is a basic characteristic of lung cancer, and indoleamine-2,3-dioxygenase （IDO） plays a key role in mediating immune escape of non-small-cell lung cancer, which leads to recurrence and metastasis. Feiji Recipe, a compound Chinese herbal medicine, has the effect of stabilizing lesions and prolonging survival in patients with lung cancer. The purpose of this study was to investigate the mechanisms underlying the anticancer properties of Feiji Recipe. Methods： An orthotopic transplant model of mouse Lewis lung cancer, with stable expression of IDO gene, was established in C57BL/6 mice. Optical imaging was used to observe the effects of Feiji Recipe in the treatment of lung cancer in vivo. The effects of Feiji Recipe on the proliferation of mouse Lewis lung cancer cell line 2LL, 2LL-enhanced green fluorescent protein （2LL-EGFP） and 2LL-EGFP-IDO were investi- gated, and the apoptosis of T-cells was examined by 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2H-tetra zolium bromide using flow cytometry. Chemical composition of Feiji Recipe was validated by high- performance liquid chromatography. Results： Compared to the control group, the survival of animals treated with Feiji Recipe was significantly prolonged （P〈0.0074）, and the IDO protein level decreased （P=0.0072）; moreover, the percentages of CD4＋CD25＋ T-cells and Foxp3＋ T-cells were significantly decreased （P〈0.05）. The molecular mechanism of Feiji Recipe against lung cancer may relate to the regulation of immune cells, such as T-cells and reg- ulatory T-cells. Conclusion： The molecular mechanism of Feiji Recipe in treatment of lung cancer is to restore the function of T-cells in the cancer microenvironment through interfering with the IDO pathway.