文献类型：期刊文献

英文题名：In vitro anti-tumor activity of 2,4′-dihydroxy-6′-methoxy-3′,5′-dimethylchalcone against six established human cancer cell lines

作者：Ye, CL; Liu, JW; Wei, DZ; Lu, YH; Qian, F

机构：[1]E China Univ Sci & Technol, Inst Biochem, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2004

卷号：50

期号：5

起止页码：505

外文期刊名：PHARMACOLOGICAL RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000224442100007)】；

语种：英文

外文关键词：Cleistocalyx operculatus; flavonoids; tumor; proliferation; apoptosis

摘要：2',4'-Dihydroxy-6'-methoxy-3',5'-dimethylchalcone (DMC), isolated from the buds of Cleistocalyx operculatus, was investigated in its cytotoxicity and its influence on six human cancer cell lines. Among SMMC-7721, 8898, HeLa, SPC-A-1, 95-D and GBC-SD cell lines, SMMC-7721 cells was the most sensitive one in these tested cell lines, with IC50 equal to 32.3 +/- 1.13 muM, EC50 equal to 9.00 +/- 0.36 muM and the therapeutic index equal to 3.59. Staining with Hoechst 33258 showed fragmentation and condensation of chromatin in the cells treated with 9 muM DMC for 48 h. Flow cytometric analysis was performed to determine hypodiploid cells. The results of flow cytometry assay indicated that the percentage of hypodiploid SMMC-7721 cells were 49.44 +/- 1.06% after 48 h treatment with 18.0 muM DMC. The treatment resulted in the appearance of a hypodiploid peak (A(0) region), probably due to the presence of apoptosing cells and/or apoptotic bodies with DNA content less than 2n. To our knowledge, this is the first report on anti-tumor activity by DMC. (C) 2004 Elsevier Ltd. All rights reserved.