文献类型：期刊文献

英文题名：Flagellin enhances the immunoprotection of formalin-inactivated Edwardsiella tarda vaccine in turbot

作者：Liu, Xiaohong[1,2];Zhang, Hua[1];Jiao, Chenglong[1];Liu, Qin[1,2,3];Zhang, Yuanxing[1,2,3];Xiao, Jingfan[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg, Shanghai 200237, Peoples R China

年份：2017

卷号：35

期号：2

起止页码：369

外文期刊名：VACCINE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000392892100026)】；

基金：This work was supported by the Project of Marine Public Welfare Profession of China (201405003-2), the National High Technology Research and Development Program of China (2014AA093604), the Modern Agro-industry Technology Research System (CARS-50-G07) and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Flagellin; Vaccine; Adjuvant; Edwardsiella tarda; Turbot

摘要：Edwardsiella tarda is an important fish pathogen that causes extensive losses in farmed fish around the world. Various types of vaccines have been developed against E. tarda infection, such as inactivated vaccine, live attenuated vaccine and subunit vaccine. However, inactivated vaccine exhibits poor immune protection against edwardsiellosis in some reports. In this study, we aimed to evaluate the protective effect of formalin-killed cells (FKC) against E. tarda with flagellin, FlgD as an adjuvant in turbot. As a result, the relative percent survival (RPS) of turbot vaccinated with FKC reached 70% in the presence of Fig) adjuvant. Moreover, specific serum antibodies reached the highest level at 3 weeks post vaccination. Furthermore, the transcription of some immune response-related genes, such as MHC-I, IgM, IL-1 beta, TCR, and TNF alpha were up-regulated in turbot after vaccination, indicating that both humoral and cellular immune responses were induced by this vaccine. In summary, flagellin can enhance immunoprotection of the formalin-inactivated E. tarda vaccine, which can be used as a potential adjuvant in fish vaccine. (C) 2016 Published by Elsevier Ltd.