文献类型：期刊文献

英文题名：Characterization of Chitin-Glucan Complex of Ganoderma lucidum Extract and Its Application as Hemostatic Hydrogel

作者：Liu, Yanqiong[1];Zhang, Chunyue[1,2];Liu, Lujie[1,2];Zhang, Xingxing[1];Hou, Yanying[1];Zhao, Liming[1,2]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol SC, Shanghai 200237, Peoples R China

年份：2022

卷号：13

期号：7

起止页码：3297

外文期刊名：WASTE AND BIOMASS VALORIZATION

收录：;EI(收录号：20220811671589);WOS:【SCI-EXPANDED(收录号:WOS:000755499500001)】；

基金：This work was supported by the National Key R&D Program of China (2019YFD0901805), the 111 Project (B18022), the Fundamental Research Funds for the Central Universities, and the Open Project Funding of the State Key Laboratory of Bioreactor Engineering, ECUST (ZDXM2019). We acknowledge Yuan Li for the assistance in performing the cytotoxicity experiments.

语种：英文

外文关键词：Ganoderma lucidum; Chitin-glucan complex; Ionic liquids; Hydrogel; Hemostatic material

摘要：Chitin and glucan in the cell wall of Ganoderma lucidum have various biological activities, but are usually not effectively utilized or discarded as insoluble waste. The aim of this study is to characterize the chitin-glucan complex extracted from Ganoderma lucidum residues and to access its application potential as a hemostatic material. Ganoderma lucidum chitin-glucan complex (GLCGC) was extracted from Ganoderma lucidum, and GLCGC was prepared into a novel Ganoderma lucidum chitin-glucan complex hydrogel (GLCGCH) with ionic liquid [AMIM]Cl. GLCGC and lyophilized GLCGCH was characterized by fourier transform infrared spectroscopy, scanning electron microscope, swelling capacity, rheological properties and hemostatic properties. Our results showed that the extracted GLCGC mainly consisted of glucan (76.7 +/- 2.9%) and chitin (3.85 +/- 0.55%). The hydrogel GLCGCH had three-dimensional porous structure with good performance in swelling behavior (with swelling ratios of 1181.0-1891.0%). With no cytotoxicity against mouse fibroblast cells, GLCGCH showed good blood compatibility (with hemolysis ratios of 0.9-1.5%) and in vitro blood coagulation property (with blood clotting indices of 35.3-41.9%), indicating GLCGCH has great hemostatic effect. This work provided new insight into the integrated utilization of Ganoderma lucidum residues. The prepared hydrogel could be used as a promising material for hemostatic application. [GRAPHICS] .