文献类型：期刊文献

英文题名：Biodegradation of penicillin G sodium by Sphingobacterium sp. SQW1: Performance, degradation mechanism, and key enzymes

作者：Zhang, Sinan[1,2];Liu, YuXuan[1];Mohisn, Ali[2];Zhang, Guohui[1];Wang, Zejian[2];Wu, Shiyong[1]

机构：[1]East China Univ Sci & Technol, Engn Res Ctr Resource Utilizat Carbon Containing W, Minist Educ, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Dept Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2024

卷号：468

外文期刊名：JOURNAL OF HAZARDOUS MATERIALS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001199465000001)】；

基金：This work was supported by the financial support of research projects from the National Key R & D Program of China (No. 2021YFC2101000) , the National Natural Science Foundation of China [No. 32071471] , the Fundamental Research Funds for the Central Universities (No. 222201714024) , and Innovation and Intelligence 111 Plan.

语种：英文

外文关键词：Penicillin G sodium; Biodegradation; Degradation products; Genomics

摘要：Biodegradation is an efficient and cost-effective approach to remove residual penicillin G sodium (PGNa) from the environment. In this study, the effective PGNa-degrading strain SQW1 (Sphingobacterium sp.) was screened from contaminated soil using enrichment technique. The effects of critical operational parameters on PGNa degradation by strain SQW1 were systematically investigated, and these parameters were optimized by response surface methodology to maximize PGNa degradation. Comparative experiments found the extracellular enzyme to completely degrade PGNa within 60 min. Combined with whole genome sequencing of strain SQW1 and LC-MS analysis of degradation products, penicillin acylase and beta-lactamase were identified as critical enzymes for PGNa biodegradation. Moreover, three degradation pathways were postulated, including beta-lactam hydrolysis, penicillin acylase hydrolysis, decarboxylation, desulfurization, demethylation, oxidative dehydrogenation, hydroxyl reduction, and demethylation reactions. The toxicity of PGNa biodegradation intermediates was assessed using paper diffusion method, ECOSAR, and TEST software, which showed that the biodegradation products had low toxicity. This study is the first to describe PGNa-degrading bacteria and detailed degradation mechanisms, which will provide new insights into the PGNa biodegradation.