文献类型：期刊文献

英文题名：AuNPs-COFs Core-Shell Reversible SERS Nanosensor for Monitoring Intracellular Redox Dynamics

作者：Chen, Zhen-Chi[1];Xu, Han-Bin[1];Chen, Hua-Ying[1];Zhu, Shi-Cheng[1];Huang, Wen-Fei[1];He, Yue[1];Hafez, Mahmoud Elsayed[2];Qian, Ruo-Can[1];Li, Da-Wei[1]

机构：[1]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem,Key Lab Adv Mat,Jo, Shanghai 200237, Peoples R China;[2]Beni Suef Univ, Fac Sci, Dept Chem, Bani Suwayf 62511, Egypt

年份：2022

卷号：94

期号：41

起止页码：14280

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20224112893468);WOS:【SCI-EXPANDED(收录号:WOS:000872958700001)】；

基金：? ACKNOWLEDGMENTS This work was financially supported by the National Natural Science Foundation of China (Grants 21788102, 21974046, 22176058, and 21977031) , Science and Technology Commis-sion of Shanghai Municipality (Grants 19391901700, 19520744000, and 19ZR1472300) , and the Fundamental Research Funds for the Central Universities. The authors thank Research Center of Analysis and Test of East China University of Science and Technology for the characterization.

语种：英文

外文关键词：Dynamics - Gold nanoparticles - Molecules - Nanosensors - Raman scattering - Raman spectroscopy - Signal transduction - Surface scattering

摘要：The redox homeostasis in living cells is greatly crucial for maintaining the redox biological function, whereas accurate and dynamic detection of intracellular redox states still remains challenging. Herein, a reversible surface-enhanced Raman scattering (SERS) nanosensor based on covalent organic frameworks (COFs) was prepared to dynamically monitor the redox processes in living cells. The nanosensor was fabricated by modifying the redox-responsive Raman reporter molecule, 2-Mercaptobenzoquione (2-MBQ), on the surface of gold nanoparticles (AuNPs), followed by the in situ coating of COFs shell. 2-MBQ molecules can repeatedly and quickly undergo reduction and oxidation when successively treated with ascorbic acid (AA) and hypochlorite (ClO-) (as models of reductive and oxidative species, respectively), which resulted in the reciprocating changes of SERS spectra at 900 cm-1. The construction of the COFs shell provided the nanosensor with great stability and anti-interference capability, thus reliably visualizing the dynamics of intracellular redox species like AA and ClO- by SERS nanosensor. Taken together, the proposed SERS strategy opens up the prospects to investigate the signal transduction pathways and pathological processes related with redox dynamics.