文献类型：期刊文献

英文题名：Ultrabright near-infrared fluorescent DNA frameworks for near-single-cell cancer imaging

作者：Liu, Xia[1,2];Shi, Ben[3,4];Gao, Yue[5];Zhu, Shitai[5];Yan, Qinglong[2];Liu, Xiaoguo[6,7];Shi, Jiye[5];Li, Qian[6,7];Wang, Lihua[1];Li, Jiang[1];Zhao, Chunchang[3,4];Tian, He[3,4];Willner, Itamar[8];Zhu, Ying[1];Fan, Chunhai[6,7,9]

机构：[1]Shanghai Univ, Inst Materiobiol, Coll Sci, Shanghai, Peoples R China;[2]Xiangfu Lab, Jiashan, Peoples R China;[3]East China Univ Sci & Technol, Key Lab Adv Mat, Shanghai, Peoples R China;[4]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn, Feringa Nobel Prize Scientist Joint Res Ctr,Inst F, Shanghai, Peoples R China;[5]Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai, Peoples R China;[6]Shanghai Jiao Tong Univ, Zhang Jiang Inst Adv Study, Frontiers Sci Ctr Transformat Mol, Sch Chem & Chem Engn, Shanghai, Peoples R China;[7]Shanghai Jiao Tong Univ, Natl Ctr Translat Med, Shanghai, Peoples R China;[8]Hebrew Univ Jerusalem, Inst Chem, Jerusalem, Israel;[9]Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Inst Mol Med,Shanghai Key Lab Nucl Acids Chem & Na, Shanghai, Peoples R China

年份：2025

卷号：19

期号：1

起止页码：79

外文期刊名：NATURE PHOTONICS

收录：;EI(收录号：20244317232336);WOS:【SCI-EXPANDED(收录号:WOS:001335890900001)】；

基金：We thank F. Zhang (Fudan University) and Q. Wang (Suzhou Institute of Nano-Tech and Nano-Bionics) for the NIR imaging assistance. This work was supported by the National Key R&D Program of China (2023YFC3404200 to Y.Z.), the National Natural Science Foundation of China (T2188102 to C.F., 21991134 to C.F., 22325406 to J.L. and 12305400 to Q.Y.), 2022 Shanghai 'Science and Technology Innovation Action Plan' Fundamental Research Project (22JC1401203 to Y.Z.) and the Xiangfu Lab Research Project (XF012022E0100 to C.F.). We would like to dedicate this paper to C.Z., who unfortunately passed away just before this paper was revised. C.Z. played an essential role in the research described here and he is greatly missed.

语种：英文

外文关键词：Infrared imaging - Mammals

摘要：Cancer imaging approaching single-cell levels is highly desirable for studying in vivo cell migration and cancer metastasis. However, current imaging probes struggle to simultaneously achieve high sensitivity, deep-tissue penetration and tissue specificity. Here we report size- and shape-resolved fluorescent DNA framework (FDF) dots with tail emission in the second near-infrared window (1,000-1,700 nm, NIR-II), which enable near-single-cell-level, tumour-targeting deep-tissue (similar to 1 cm) NIR-II imaging in tumour-bearing mouse models. The construction of DNA frameworks with embedded hydrophobic nanocavity results in the non-covalent encapsulation of a designed NIR-Ib (900-1,000 nm) probe (dye Sq964). The FDF dots exhibit high water solubility, brightness and photostability. We find that the stable tumour retention of FDF dots with enhanced signal intensity arises from their shape-dependent accumulation in tumour cells. FDF-dot-based cancer imaging reveals in vivo sensitivity down to similar to 40 tumour cells, high tumour-to-normal tissue ratios up to similar to 26 and long-term imaging over 11 days. We also demonstrate NIR-II-image-guided breast cancer surgery with the complete excision of metastases with a minimum size of similar to 53 mu m (similar to 20 cells).