文献类型：期刊文献

英文题名：Multi-Scale Analysis of Nickel Tolerance Mechanism for Thermophilic Sulfobacillus Thermosulfidooxidans in Bioleaching

作者：Chen, Guanglin[1]; Shi, Hongjie[1]; Ding, Huili[1]; Zhang, Xu[1]; Gu, Tingyue[2]; Zhu, Minglong[1]; Tan, Wensong[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China; [2] Department of Chemical and Biomolecular Engineering, Ohio University, Athens, OH, United States

年份：2022

外文期刊名：SSRN

收录：EI(收录号：20220324317)

语种：英文

外文关键词：Adenosinetriphosphate - Cell proliferation - Energy utilization - Genes - Metabolism - Metal ions - Nickel

摘要：The possible mechanisms underlying the response of S. thermosulfidooxidans to nickel ion stress was analyzed using a multi-scale method. Under the condition of nickel ion stress, high concentrations of nickel ions were immobilized by extracellular polymeric substances, while low concentrations of nickel ions appeared inside the cells. The intracellular adenosine triphosphate (ATP) concentration and H+-ATPase activity increased to maintain normal cell growth and metabolic activities. Scavenging abilities of S. thermosulfidooxidans for hydrogen peroxide and superoxide anion were enhanced to reduce oxidative damage induced by nickel ion stress. There were 734 differentially expressed genes identified by RNA-seq under nickel ion stress. Most of them were involved in oxidative phosphorylation, glutathione metabolism and genetic information processing, responsible for intracellular energy utilization, intracellular antioxidant capacity and DNA damage repair, respectively. The results of this study are of major significance for in-depth understanding of the mechanism of acidophilic microorganisms’ resistance to metal ions. ? 2022, The Authors. All rights reserved.