文献类型：期刊文献

中文题名：柠檬酸钠对枯草杆菌生长代谢及肌苷积累的影响

英文题名：Effect of Sodium Citrate on The Growth Metabolism and Inosine Accumulation by Bacillus subtilis

作者：刘新星[1];陈双喜[1];储炬[1];庄英萍[1];张嗣良[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2004

卷号：44

期号：5

起止页码：627

中文期刊名：微生物学报

外文期刊名：Acta Microbiologica Sinica

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；PubMed;

基金：上海市曙光计划资助项目 ( 0 1SG2 8)~~

语种：中文

中文关键词：柠檬酸钠;枯草杆菌;生长;代谢;肌苷;丙酮酸激酶;糖酵解通量

外文关键词：Inosine, Sodium citrate, Pyruvate kinase, Glycolysis flux

摘要：研究柠檬酸钠对枯草杆菌生长代谢及产苷的影响 ,在基础料中添加浓度为 0 2g L的柠檬酸钠 ,肌苷产量提高 18% ,肌苷对葡萄糖得率增加 38%。通过分析糖代谢途径中关键酶的酶活 ,结果表明添加柠檬酸钠改变了一些关键酶的活力 ,可降低糖酵解途径中 6_磷酸果糖激酶和丙酮酸激酶的活力 ,从而减弱了糖酵解途径的通量。
The effects of sodium citrate on the growth metabolism and inosine accumulation by Bacillus subtillus were studied in this paper.When 0.2% sodium citrate was added into the basal medium,the inosine yield and the yield coefficient of inosine to glucose in the broth were increased 18% and 38% respectively.The analysis on the activities of some key enzymes during inosine fermentation revealed that the supplement of sodium citrate had profound effect on the activity of several key enzymes. The activities of Phosphofructokinase and Pyruvate Kinase were decreased remarkably,the flux of glycolysis pathway was thus cut down as well.