文献类型：期刊文献

英文题名：Ratiometric sensing of β-galactosidase based on excited-state intramolecular proton transfer (ESIPT) and solid-state luminescence enhancement

作者：Tian Jr, He[1];Lin, Wei[1];Hu, Xi-Le[1];Wang, Jing-Bo[1];Zhang, Min-Yu[1];Zang, Yi[2,6,7];Wu, Xin-Yan[1];Li, Jia[2,6];James, Tony D.[4,5];He, Xiao-Peng[1,3]

机构：[1]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Key Lab Adv Mat & Joint Int Res Lab Precis Chem &, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, State Key Lab Drug Res, Shanghai 201203, Peoples R China;[3]Eastern Hepatobiliary Surg Hosp, Natl Ctr Liver Canc, Int Cooperat Lab Signal Transduct, Shanghai 200438, Peoples R China;[4]Univ Bath, Dept Chem, Bath BA2 7AY, England;[5]Henan Normal Univ, Sch Chem & Chem Engn, Xinxiang 453007, Peoples R China;[6]Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou 310024, Peoples R China;[7]Lingang Lab, Shanghai 201203, Peoples R China

年份：2023

卷号：10

期号：12

起止页码：2913

外文期刊名：ORGANIC CHEMISTRY FRONTIERS

收录：;EI(收录号：20232214170107);WOS:【SCI-EXPANDED(收录号:WOS:000988145000001)】；

基金：The authors thank the Natural National Science Foundation of China (no. 91853201, 92253306 and 82151219), the Shanghai Municipal Science and Technology Major Project (no. 2018SHZDZX03), the Fundamental Research Funds for the Central Universities (222201717003), the Programme of Introducing Talents of Discipline to Universities (B16017) and the Open Funding Project of the State Key Laboratory of Bioreactor Engineering. TDJ wishes to thank the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University for support (2020ZD01). The Research Center of Analysis and Test of East China University of Science and Technology is gratefully acknowledged for assistance in analytical experiments.

语种：英文

外文关键词：Diagnosis - Excited states - Fluorescence - Probes - Proton transfer

摘要：Glycosidases play important roles in modulating the structural and functional integrity of glycoproteins and glycolipids, and thus are promising biomarkers for disease diagnosis. While current approaches for glycosidase detection mainly rely on an enhancement of the UV-vis absorbance or fluorescence emission of glycosyl indicators, here we develop a ratiometric fluorescent probe for the sensitive and selective detection of glycosidase activity based on the combined mechanisms of excited-state intramolecular proton transfer (ESIPT) and solid-state luminescence enhancement (SSLE). The probe behaves like a typical SSLE when glycosylated, and exhibits a similar to 140 nm red-shift in fluorescence owing to activation of ESIPT after deglycosylation. Such a large Stokes shift may facilitate the unbiased analysis of glycosidase activities when used in diagnostic and drug-screening assays.