文献类型：期刊文献

英文题名：Impact of a novel precursor on FK506 production and key gene transcription in Streptomyces tsukubaensis No. 9993

作者：Sun, Yan[1];Ye, Ruifang[1]

机构：[1]E China Univ Sci & Technol, Dept Bioengn, Shanghai 200237, Peoples R China

年份：2016

卷号：42

期号：4

起止页码：3351

外文期刊名：RESEARCH ON CHEMICAL INTERMEDIATES

收录：;EI(收录号：20153801283129);WOS:【SCI-EXPANDED(收录号:WOS:000372159200040)】；

语种：英文

外文关键词：FK506; Salicyl alcohol; RT-qPCR; Gene transcription; Fermentation

摘要：FK506, a widely used immunosuppressant, is produced by industrial fermentation using Streptomyces tsukubaensis. In this study, a novel precursor, salicyl alcohol, was added and provided significant improvement in FK506 production. Furthermore, differences of the expression of the structural genes between the two media were also investigated. The results showed that the expression of key genes in enriched medium was much higher than that in the control. Compared with the non-enriched medium, the enriched medium caused 52.25-% enhancement of FK506 production. Salicyl alcohol could play a crucial role in the transcription of FK506 structural genes.