文献类型：期刊文献

英文题名：Gemfibrozil reduces lipid accumulation in SMMC-7721 cells via the involvement of PPAR and SREBP1

作者：Zhang, Xiaonan[1];Wang, Song[1];Hu, Linlin[1];Wang, Jian[1];Liu, Yajing[1];Shi, Ping[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2019

卷号：17

期号：2

起止页码：1282

外文期刊名：EXPERIMENTAL AND THERAPEUTIC MEDICINE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000455641300043)】；

基金：The present work was sponsored by grants from Shanghai Scientific and Technological Innovation Project (grant no. 14520720700) and State Education Ministry and Fundamental Research Funds for the Central Universities (grant no. 222201313010).

语种：英文

外文关键词：human hepatoma SMMC-7721 cells; oleic acid; gemfibrozil

摘要：Gemfibrozil (GEM) is a member of the fibrate class of lipid-lowering pharmaceuticals and has been widely used in the therapy of different forms of hyperlipidemia and hypercholesterolemia. Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease and is becoming an important public health concern worldwide. However, there is little knowledge about the effects of GEM on NAFLD. In the present study, oleate-treated human hepatoma SMMC-7721 cells were utilized to investigate the role of GEM in regulating hepatic lipid metabolism. The present results demonstrated that GEM attenuated excessive intracellular triglyceride content in the steatosis model. Upregulation of peroxisome proliferator-activated receptor (PPAR) protein and sterol regulatory element-binding protein 1 (SREBP1) was detected following treatment with GEM. Additionally, reverse transcription-polymerase chain reaction analysis demonstrated that GEM increased the downstream genes related to PPAR and SREBP1, including carnitine palmitoyltransferase 2, acyl-coA oxidase 1, hydroxyacyl-CoA dehydrogenase, LIPIN1 and diacylglycerol O-acyltransferase 1. These findings demonstrated that GEM alleviated hepatic steatosis via the involvement of the PPAR and SREBP1 signaling pathways, which enhances lipid oxidation and interferes with lipid synthesis and secretion. Taken together, the data provide direct evidence that GEM may lower lipid accumulation in hepatocellular steatosis cells in vitro and that it may have a potential therapeutic use for NAFLD.