文献类型：期刊文献

英文题名：Development of an All-Solid-State Silver Ion-Selective Electrode Based on the Polypyrrole/Multiwalled Carbon Nanotubes for Thrombin Detection

作者：Jiang, Dong[1];Tu, Feng[2];Wang, Qingjiang[2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]East China Normal Univ, Sch Chem & Mol Engn, Shanghai 200241, Peoples R China

年份：2026

卷号：26

期号：10

起止页码：14998

外文期刊名：IEEE SENSORS JOURNAL

收录：;EI(收录号：20261420404313);WOS:【SCI-EXPANDED(收录号:WOS:001765020400010)】；

基金：This work was supported by the National Natural Science Foundation of China under Grant 22074042.

语种：英文

外文关键词：Electrodes; Electric potential; Carbon nanotubes; Silver; Plastics; Sensors; Ions; Nanoparticles; Magnetic separation; Silicon compounds; Ag+; all-solid-state ion-selective electrode (ISE); polypyrrole/multiwalled carbon nanotubes (MWCNTs) nanocomposite; thrombin

摘要：All-solid-state ion-selective electrodes (ISEs) have been rapidly developed in recent years due to their advantages of simple fabrication, fast response, and ease of miniaturization and array formation. However, issues, including low ion-electron conversion efficiency at the interface between the electrode substrate and the selective membrane, small double-layer capacitance, and the formation of a water layer, may lead to poor potential stability. This work developed an all-solid-state Ag+-ISE utilizing a polypyrrole/multiwalled carbon nanotubes (PPY-MWCNTs) nanocomposite as the solid-contact layer. By combining the excellent conductivity of polypyrrole with the high capacitance of carbon nanotubes, the developed Ag+-ISE effectively enhanced ion-electron transfer while suppressing water layer formation, thereby exhibiting the outstanding potential stability. It exhibited a linear response to Ag+ in the concentration range of 1.0 & times; 10(-6)-1.0 & times; 10(-2) mol/L, with a slope of 48.0 +/- 1.7 mV/dec and a detection limit of 7.1 & times; 10(-7 )mol/L. Relying on the readily oxidizable nature of silver nanoparticles (Ag NPs), an indirect detection strategy has been proposed for thrombin. Through the specific recognition between thrombin and its two aptamers (Aptamers 15 and 29), a sandwich-type nanocomplex (Fe3O4-Apt 15/thrombin/Apt 29-Ag@SiO2) was constructed. Following magnetic separation, Ag NPs within the nanocomplex were oxidized by nitric acid to release Ag+. The prepared PPY-MWCNTs/Ag+-ISE then quantitatively detected Ag+ via opencircuit potential measurement, thereby enabling the detection of thrombin. Benefiting from the signal amplification effect of Ag@SiO2, this electrode exhibited a detection sensitivity of 24.1 mV/dec and a detection limit of 4.2 UN/mL for thrombin, showing a high potential for application to other biomarkers.