文献类型：期刊文献

英文题名：A highly-efficient isothermal nano-detection platform coupling CRISPR/Cas technology for detection of circRNA

作者：Sun, Kexin[1];Wu, Haizhen[1,2,3]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Sch Biotechnol, Dept Appl Biol, Shanghai, Peoples R China;[3]East China Univ Sci & Technol, Fermentat Engn Expt Teaching Demonstrat Ctr, Shanghai 200237, Peoples R China

年份：2026

卷号：151

期号：5

起止页码：1304

外文期刊名：ANALYST

收录：;EI(收录号：20260820128189);WOS:【SCI-EXPANDED(收录号:WOS:001693152200001)】；

基金：This work was supported by the National Key Research and Development Program of China (2021YFC2100600) and the National Natural Science Foundation of China (NSFC) (31900059).

语种：英文

外文关键词：Biomarkers - Diagnosis - Diseases - Pathology - Signal processing

摘要：Triple-negative breast cancer (TNBC), an aggressive molecular subtype of breast cancer with poor prognosis, is characterized by a high rate of metastasis and proliferation, which makes early detection particularly challenging. Early diagnosis of TNBC through biomarkers and prompt development of treatment methods can lower its mortality rate. This work has designed a nano-detection platform for TNBC biomarker circRNA based on the CRISPR/Cas system and isothermal amplification strategy. Specifically, this detection system uses functional nucleic acid molecules for recognition of circCD44, as well as dual signal amplification using Klenow(3 '-5 ' exo-) and Cas9n. Furthermore, it combines Cas12a and immunomagnetic beads for an extra signal boost and output. After confirming its feasibility and optimizing the conditions, the detection system achieved a 1.73-fold enhancement in sensitivity, offering a linear detection range of 1 pM to 100 nM, with the limit of detection lowered to 95.1 fM. It also showed good specificity through testing against 5 biomarkers. Therefore, this detection system provides a novel strategy for the early diagnosis of TNBC and other diseases.