文献类型：期刊文献

英文题名：Continuous culture and proteomic analysis of Escherichia coli DH5α and its acetate-tolerant mutant DA19 under conditions of nitrogen source limitation

作者：Zhang, Xiaoyun[1,2];Zhang, Yanjun[1];Li, Zhimin[1];Xia, Yuelan[1];Ye, Qin[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang, Peoples R China

年份：2011

卷号：34

期号：2

起止页码：179

外文期刊名：BIOPROCESS AND BIOSYSTEMS ENGINEERING

收录：;EI(收录号：20110513636892);WOS:【SCI-EXPANDED(收录号:WOS:000286190600007)】；

基金：This work was partially supported by the National Special Fund for State Key Laboratory of Bioreactor Engineering, Grant No. 2060204 and the project of Shanghai Leading Academic Disciplines (No. B505).

语种：英文

外文关键词：Proteomics; Escherichia coli; Acetic acid; Electrophoresis; Continuous culture

摘要：Escherichia coli DH5 alpha grows poorly in a chemically defined medium, but its acetate-tolerant mutant, DA19, grows fast and produces less acetate. To investigate the differences in metabolism between the two strains, the samples of continuous cultures at steady state were analyzed by two-dimensional SDS-PAGE (2-D SDS-PAGE) and peptide mass fingerprinting (PMF). The results indicated that in DA19, expression of gnd, yddS, and purH (except for adenine supplement) was up-regulated, and expression of atpA, glnK, and ompR, which encode membrane proteins, was significantly up-regulated. Expression of ackA and yahK in DA19 was down-regulated. In the presence of acetate, hchA and ydcW in DH5 alpha were significantly down-regulated. This study shed light on the differences in metabolic properties between DH5 alpha and DA19, and provided some ideas for improving strains through metabolic engineering.